FastQCFastQC Report
Fri 18 Apr 2025
Yell_July18_Site1m_S102_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYell_July18_Site1m_S102_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167884
Total Bases50.5 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC3054018.191131972075958No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC2531315.077672678754379No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC1866611.11839127016273No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC76704.568630721212266No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC61633.6709871101474825No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC42692.542827190202759No Hit
TGATCCTTCTGCAGGTTCACCTACAGATACCTTGTTACGACTTAACCCTC14320.8529699077934765No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTGTCCTTC10690.6367491839603535No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTCCTTC6790.4044459269495604No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCACCTTC6490.3865764456410378No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6050.3603678730552048No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTTTACTTC5430.32343761168425816No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGCCTTATCCTTC5210.3103333253913417No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTAGTTC4910.2924638440828191No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG4780.2847204021824593No Hit
TAGACCTCGGGGTGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG4160.2477901408115127No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCTCCTTC4050.24123799766505444No Hit
TAGACTTCGGGGTGGCCGAAAAATCAAAATAAATGTTGGTATAACACTGG3830.22813371137213792No Hit
TAGACTTCGGGGTGGCCAAAGAATCAAAATAAATGTTGGTATAACACTGG3640.21681637321007363No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3440.20490338567105856No Hit
TAGACTTCGGGATGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG3210.1912034500011913No Hit
TAGACCTCGGGGTGGCCGAAAAATCAAAATAAATGTTGGTATAACACTGG3010.17929046246217625No Hit
TAGACCTCGGGGTGGCCAAAGAATCAAAATAAATGTTGGTATAACACTGG2950.17571656620047174No Hit
TAGACCTCGGGATGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG2800.1667818255462105No Hit
TAGACTTCGGGATGGCCGAAAAATCAAAATAAATGTTGGTATAACACTGG2600.15486883800719545No Hit
TAGACTTCGGGGTGGCCGAAGAACCAAAATAAATGTTGGTATAACACTGG2560.15248624049939244No Hit
TGATCCTTCTTCAGGTTCACCTACAGATACCTTGTTACGACTTAACCCTC2550.1518905911224417No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTGTCCTTC2540.15129494174549094No Hit
TAGACTTCGGGATGGCCAAAGAATCAAAATAAATGTTGGTATAACACTGG2390.14236020109122965No Hit
TAGACCTCGGGGTGGCCGAAGAACCAAAATAAATGTTGGTATAACACTGG2360.1405732529603774No Hit
TAGACCTCGGGATGGCCGAAAAATCAAAATAAATGTTGGTATAACACTGG2210.13163851230611612No Hit
TAGACTTCGGGGTGGCCAAAAAATCAAAATAAATGTTGGTATAACACTGG2180.12985156417526386No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCATC2080.12389507040575636No Hit
TAGACTTCGGGGTGGCCGAAAAACCAAAATAAATGTTGGTATAACACTGG2050.1221081222749041No Hit
TAGACTTCGGTGTGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG1960.11674727788234733No Hit
TGATCCTTCTGCAGGTTCACCTACGGCTACCTTGTTACGACTTCACCCCA1920.11436468037454434No Hit
TAGACTTCGGGATGGCCGAAGAACCAAAATAAATGTTGGTATAACACTGG1900.11317338162064282No Hit
TAGACCTCGGTGTGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG1890.11257773224369208No Hit
TAGACCTCGGGGTGGCCAAAAAATCAAAATAAATGTTGGTATAACACTGG1870.11138643348979056No Hit
TGATCCTTCTGCAGGTTCACCTACAGCAACCTTGTTACGACTTTTGCTTC1800.10721688785113531No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)