FastQCFastQC Report
Fri 18 Apr 2025
Yell_July18_Site1k_S100_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYell_July18_Site1k_S100_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116323
Total Bases35 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC2846324.468935636116676No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC1312511.283237192988489No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC94728.142843633675199No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC46463.994051047514249No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC21941.8861274210603234No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC15401.3238998306439826No Hit
TGATCCTTCTGCAGGTTCACCTACAGATACCTTGTTACGACTTAACCCTC6070.5218228553252581No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6060.5209631801105542No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTGTCCTTC5920.5089277271046999No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTTTACTTC4880.4195215047754958No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTCCTTC3810.3275362568021801No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG3250.27939444477876263No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTAGTTC3110.2673589917729082No Hit
TAGACCTCGGGGTGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG2680.23039295754064115No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2590.22265588060830618No Hit
TAGACTTCGGGGTGGCCGAAAAATCAAAATAAATGTTGGTATAACACTGG2570.22093653017889842No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAATAAATGCTGGTACAGCACTGG2540.21835750453478675No Hit
TAGACCTCGGGGTGGCCGAAGAATCAAAATAAATGCTGGTACAGCACTGG2510.21577847889067509No Hit
TAGACTTCGGGGTGGCCAAAGAATCAAAATAAATGTTGGTATAACACTGG2390.20546237631422848No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCACCTTC2370.2037430258848207No Hit
TAGACTTCGGGATGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG2190.18826887202015077No Hit
TAGACCTCGGGGTGGCCGAAAAATCAAAATAAATGTTGGTATAACACTGG2090.17967211987311194No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAATAAGTGTTGATAGAGAATAGG2090.17967211987311194No Hit
TAGACTTCGGGATGGCCGAAAAATCAAAATAAATGTTGGTATAACACTGG2050.1762334190142964No Hit
TAGACCTCGGGGTGGCCAAAGAATCAAAATAAATGTTGGTATAACACTGG1960.16849634208196143No Hit
TAGACTTCGGGATGGCCGAAGAATCAAAATAAATGCTGGTACAGCACTGG1950.16763666686725756No Hit
TAGACTTCGGGGTGGCCGAAAAATCAAAATAAATGCTGGTACAGCACTGG1910.164197966008442No Hit
TAGACTTCGGGGTGGCCGAAGAACCAAAATAAATGTTGGTATAACACTGG1900.16333829079373813No Hit
TAGACCTCGGGATGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG1870.16075926514962646No Hit
TAGACTTCGGGATGGCCAAAGAATCAAAATAAATGTTGGTATAACACTGG1680.14442543607025266No Hit
TAGACTTCGGGGTGGCCAAAGAATCAAAATAAATGCTGGTACAGCACTGG1660.1427060856408449No Hit
TAGACCTCGGGGTGGCCGAAGAACCAAAATAAATGTTGGTATAACACTGG1650.141846410426141No Hit
TAGACCTCGGGGTGGCCGAAGAATCAAAATAAGTGTTGATAGAGAATAGG1630.14012705999673325No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCTCCTTC1610.13840770956732548No Hit
TAGACCTCGGGGTGGCCGAAAAATCAAAATAAATGCTGGTACAGCACTGG1600.13754803435262158No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAGCCTTGTTACGACTTCTCCTTC1590.1366883591379177No Hit
TAGACTTCGGGGTGGCCAAAAAATCAAAATAAATGTTGGTATAACACTGG1580.1358286839232138No Hit
TAGACTTCGGGGTGGCCGAAAAATCAAAATAAGTGTTGATAGAGAATAGG1570.13496900870850992No Hit
TAGACTTCGGGGTGGCCGAAGAACCAAAATAAATGCTGGTACAGCACTGG1560.13410933349380605No Hit
TAGACTTCGGGATGGCCGAAGAATCAAAATAAGTGTTGATAGAGAATAGG1530.13153030784969438No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAAAAGGTGCTGGAACAAAATAGG1500.12895128220558272No Hit
TAGACCTCGGGATGGCCGAAGAATCAAAATAAATGCTGGTACAGCACTGG1500.12895128220558272No Hit
TAGACTTCGGGGTGGCCAAAGAACCAAAATAAATGTTGGTATAACACTGG1430.12293355570265553No Hit
TAGACTTCGGGGTGGCCGAAAAACCAAAATAAATGTTGGTATAACACTGG1420.12207388048795166No Hit
TAGACTTCGGGATGGCCAAAGAATCAAAATAAATGCTGGTACAGCACTGG1420.12207388048795166No Hit
TAGACCTCGGGGTGGCCAAAGAATCAAAATAAGTGTTGATAGAGAATAGG1400.12035453005854388No Hit
TAGACTTCGGGGTGGCCAAAGAATCAAAATAAGTGTTGATAGAGAATAGG1400.12035453005854388No Hit
TAGACTTCGGGATGGCCGAAAAATCAAAATAAATGCTGGTACAGCACTGG1360.11691582919972833No Hit
TAGACCTCGGGGTGGCCAAAGAATCAAAATAAATGCTGGTACAGCACTGG1360.11691582919972833No Hit
TAGACCTCGGGGTGGCCGAAAAATCAAAATAAGTGTTGATAGAGAATAGG1330.11433680355561668No Hit
TAGACCTCGGGATGGCCGAAGAACCAAAATAAATGTTGGTATAACACTGG1330.11433680355561668No Hit
TAGACCTCGGGATGGCCGAAAAATCAAAATAAATGTTGGTATAACACTGG1320.11347712834091281No Hit
TAGACCTCGGGGTGGCCAAAAAATCAAAATAAATGTTGGTATAACACTGG1320.11347712834091281No Hit
TAGACCTCGGGATGGCCAAAGAATCAAAATAAATGTTGGTATAACACTGG1320.11347712834091281No Hit
TAGACTTCGGGGTGGCCGAAGAATCAGAATAAATGTTGATATAAAATAGG1300.11175777791150503No Hit
TAGACCTCGGGGTGGCCGAAGAACCAAAATAAATGCTGGTACAGCACTGG1280.11003842748209727No Hit
TAGACCTCGGGATGGCCGAAGAATCAAAATAAGTGTTGATAGAGAATAGG1280.11003842748209727No Hit
TAGACTTCGGGGTGGCCAAAAAATCAAAATAAATGCTGGTACAGCACTGG1250.10745940183798561No Hit
TAGACTTCGGGATGGCCGAAGAACCAAAATAAATGTTGGTATAACACTGG1240.10659972662328172No Hit
TAGACTTCGGGATGGCCAAAGAATCAAAATAAGTGTTGATAGAGAATAGG1200.10316102576446617No Hit
TAGACTTCGGGGTGGCCGAAAAACCAAAATAAATGCTGGTACAGCACTGG1180.10144167533505842No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)