FastQCFastQC Report
Fri 18 Apr 2025
Yell_July18_Site1h_S94_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYell_July18_Site1h_S94_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73412
Total Bases22 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC1882025.636135781616087No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC1272617.33504059281861No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC58848.015038413338418No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC33154.515610526889337No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC21682.953195662834414No Hit
TGATCCTTCTGCAGGTTCACCTACAGCAACCTTGTTACGACTTTTGCTTC14071.9165803955756553No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC9511.29542854029314No Hit
TGATCCTTCTGCAGGTTCACCTACGGAGACCTTGTTACGACTTCTCCTTC8831.2028006320492561No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCATC7991.0883779218656349No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTGTCCTTC7791.0611344194409633No Hit
TGATCCTTCTGCAGGTTCACCTACAGATACCTTGTTACGACTTAACCCTC7701.0488748433498611No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTTTACTTC6580.8963112297716994No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCACCTTC4390.5979948782215442No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTAGTTC4020.5475943987359014No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCTCCTTC2740.3732359832180025No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTCCTTC2560.3487168310357979No Hit
TGATCCTTCTTCAGGTTCACCTACAGCAACCTTGTTACGACTTTTGCTTC2400.3269220290960606No Hit
TGATCCTTCTGCAGGTTCACCTACGAAAACCTTGTTACGACTTTTACTTC2040.2778837247316515No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2030.2765215496104179No Hit
TGATCCTTCTTCAGGTTCACCTACGGAGACCTTGTTACGACTTCTCCTTC1530.20841279354873862No Hit
TGATCCTTCTGCAGGTTCACCTACGGCTACCTTGTTACGACTTCACCCCA1470.2002397428213371No Hit
TAGACTTCGGGGTGGCCGAAGAATCAGAATAAGTGTTGGTATAGAATAGG1340.1825314662453005No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTGTCCTTC1320.17980711600283333No Hit
TAGACCTCGGGGTGGCCGAAGAATCAGAATAAGTGTTGGTATAGAATAGG1320.17980711600283333No Hit
TGATCCTTCTTCAGGTTCACCTACAGAAACCTTGTTACGACTTTTACTTC1060.1443905628507601No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCATC1050.14302838772952653No Hit
TGATCCTTCTTCAGGTTCACCTACAGATACCTTGTTACGACTTAACCCTC1050.14302838772952653No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1040.14166621260829293No Hit
TAGACTTCGGGGTGGCCGAAAAATCAGAATAAGTGTTGGTATAGAATAGG900.12259576091102273No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTGCTTC890.12123358578978913No Hit
TAGACCTCGGGATGGCCGAAGAATCAGAATAAGTGTTGGTATAGAATAGG870.11850923554732197No Hit
TAGACCTCGGGGTGGCCAAAGAATCAGAATAAGTGTTGGTATAGAATAGG830.11306053506238763No Hit
TAGACCTCGGGGTGGCCGAAAAATCAGAATAAGTGTTGGTATAGAATAGG820.11169835994115404No Hit
TAGACTTCGGGGTGGCCAAAGAATCAGAATAAGTGTTGGTATAGAATAGG820.11169835994115404No Hit
TAGACCTCGGGGTGGCCGAAGAACCAGAATAAGTGTTGGTATAGAATAGG750.10216313409251894No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)