FastQCFastQC Report
Fri 18 Apr 2025
Yell_July18_Site1e_S91_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYell_July18_Site1e_S91_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30883
Total Bases9.2 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC548717.76705630929638No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC443214.35093740893048No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC17985.821973253893728No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC12073.908299064210083No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC9413.0469837774827577No Hit
TGATCCTTCTGCAGGTTCACCTACGGAGACCTTGTTACGACTTCTCCTTC5371.73882071042321No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC3671.1883560534922126No Hit
TGATCCTTCTGCAGGTTCACCTACAGCAACCTTGTTACGACTTTTGCTTC3271.0588349577437424No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTGTCCTTC2130.6896998348606029No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTCCTTC1670.5407505747498623No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCATC1480.4792280542693391No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTTTACTTC1250.40475342421396887No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1210.3918013146391218No Hit
TGATCCTTCTTCAGGTTCACCTACGGAGACCTTGTTACGACTTCTCCTTC1100.3561830133082926No Hit
TGATCCTTCTGCAGGTTCACCTACGAAAACCTTGTTACGACTTTTACTTC1100.3561830133082926No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCACCTTC1040.3367548489460221No Hit
TAGACTTCGGGGTGGCCGAAGAATCAGAATAAGTGTTGGTATAGAATAGG900.2914224654340576No Hit
TGATCCTTCTTCAGGTTCACCTACAGCAACCTTGTTACGACTTTTGCTTC680.22018586277239904No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG620.20075769841012853No Hit
TGATCCTTCTGCAGGTTCACCTACAGATACCTTGTTACGACTTAACCCTC570.1845675614415698No Hit
TAGACCTCGGGGTGGCCGAAGAATCAGAATAAGTGTTGGTATAGAATAGG560.18132953404785804No Hit
TAGACCTCGGGATGGCCGAAGAATCAGAATAAGTGTTGGTATAGAATAGG470.1521872875044523No Hit
TAGACTTCGGGGTGGCCGAAAAATCAGAATAAGTGTTGGTATAGAATAGG450.1457112327170288No Hit
TAGACTTCGGGATGGCCGAAGAATCAGAATAAGTGTTGGTATAGAATAGG440.14247320532331703No Hit
TAGACTTCGGGGTGGCCAAAGAATCAGAATAAGTGTTGGTATAGAATAGG410.13275912314218177No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCTCCTTC410.13275912314218177No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCATC410.13275912314218177No Hit
TAGACTTCGGGGTGGCCGAAGAACCAGAATAAGTGTTGGTATAGAATAGG400.12952109574847No Hit
TAGACCTCGGGGTGGCCGAAAAATCAGAATAAGTGTTGGTATAGAATAGG360.11656898617362302No Hit
TAGACCTCGGGGTGGCCGAAGAATCAGTTCCCTGTCTCTTATACACATCT360.11656898617362302No Hit
TAGACCTCGGGGTGGCCGAAGAACCAGAATAAGTGTTGGTATAGAATAGG350.11333095877991128No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTGTCCTTC340.11009293138619952No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTTTCCTTC340.11009293138619952No Hit
TAGACCTCGGGGTGGCCAAAGAATCAGAATAAGTGTTGGTATAGAATAGG340.11009293138619952No Hit
TAGACCTCGGGATGGCCAAAGAATCAGAATAAGTGTTGGTATAGAATAGG340.11009293138619952No Hit
TAGACTTCGGGGTGGCCGAAGAATCAGTTCCCTGTCTCTTATACACATCT330.10685490399248776No Hit
TAGACTTCGGGGTGGCCAAAAAATCAGAATAAGTGTTGGTATAGAATAGG330.10685490399248776No Hit
TAGACTTCGGGATGGCCGAAAAATCAGAATAAGTGTTGGTATAGAATAGG320.10361687659877603No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTGCTTC320.10361687659877603No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)