FastQCFastQC Report
Fri 18 Apr 2025
Yell_July18_Site1d_S86_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYell_July18_Site1d_S86_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences175992
Total Bases52.9 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC3216718.27753534251557No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC3188418.11673257875358No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC1811610.293649711350517No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC49542.8149006773035135No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC49412.8075139779080867No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC26631.513136960770944No Hit
TGATCCTTCTGCAGGTTCACCTACAGATACCTTGTTACGACTTAACCCTC25891.47108959498159No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTCCTTC14850.8437883540160916No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTGTCCTTC11030.6267330333196963No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCACCTTC10740.610255011591436No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTTTACTTC9280.527296695304332No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCTCCTTC8050.45740715487067596No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTACCTTC7690.43695167962180104No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6600.3750170462293741No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG6020.34206100277285334No Hit
TAGACCTCGGGGTGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG5420.3079685440247284No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTAGTTC5270.2994454293376972No Hit
TAGACTTCGGGGTGGCCGAAAAATCAAAATAAATGTTGGTATAACACTGG4290.24376108004909317No Hit
TAGACTTCGGGGTGGCCAAAGAATCAAAATAAATGTTGGTATAACACTGG4180.23751079594527022No Hit
TGATCCTTCTGCAGGTTCACCTACGGATACCTTGTTACGACTTTTACGTC3920.2227373971544161No Hit
TAGACTTCGGGATGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG3810.2164871130505932No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3580.20341833719714533No Hit
TAGACCTCGGGGTGGCCAAAGAATCAAAATAAATGTTGGTATAACACTGG3570.20285012955134327No Hit
TAGACCTCGGGATGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG3370.19148597663530162No Hit
TAGACTTCGGGGTGGCCGAAGAACCAAAATAAATGTTGGTATAACACTGG3330.18921314605209327No Hit
TAGACCTCGGGGTGGCCGAAAAATCAAAATAAATGTTGGTATAACACTGG3280.1863721078230829No Hit
TAGACCTCGGGGTGGCCGAAGAACCAAAATAAATGTTGGTATAACACTGG2970.16875767080321832No Hit
TGATCCTTCTTCAGGTTCACCTACAGATACCTTGTTACGACTTAACCCTC2970.16875767080321832No Hit
TAGACTTCGGGATGGCCGAAAAATCAAAATAAATGTTGGTATAACACTGG2880.1636438019909996No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCATC2750.15625710259557252No Hit
TAGACTTCGGGGTGGCCAAAAAATCAAAATAAATGTTGGTATAACACTGG2640.15000681849174963No Hit
TAGACTTCGGGATGGCCAAAGAATCAAAATAAATGTTGGTATAACACTGG2550.1448929496795309No Hit
TAGACCTCGGGATGGCCGAAAAATCAAAATAAATGTTGGTATAACACTGG2510.14262011909632258No Hit
TAGACTTCGGGGTGGCCAAAGAACCAAAATAAATGTTGGTATAACACTGG2510.14262011909632258No Hit
TGATCCTTCTGCAGGTTCACCTACGAAAACCTTGTTACGACTTTTACTTC2500.14205191145052048No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAGCCTTGTTACGACTTCTCCTTC2500.14205191145052048No Hit
TAGACTTCGGGGTGGCCGAAAAACCAAAATAAATGTTGGTATAACACTGG2450.13921087322151005No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTTTCCTTC2310.13125596618028093No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTTACCTTC2310.13125596618028093No Hit
TGATCCTTCTGCAGGTTCACCTACGGAGACCTTGTTACGACTTCTCCTTC2170.12330105913905179No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTATGACTTCTCCTTC2110.1198918132642393No Hit
TAGACCTCGGGATGGCCGAAGAACCAAAATAAATGTTGGTATAACACTGG2070.11761898268103095No Hit
TAGACCTCGGGGTGGCCAAAAAATCAAAATAAATGTTGGTATAACACTGG2060.11705077503522887No Hit
TAGACCTCGGGATGGCCAAAGAATCAAAATAAATGTTGGTATAACACTGG2060.11705077503522887No Hit
TAGACTTCGGGATGGCCGAAGAACCAAAATAAATGTTGGTATAACACTGG1950.11080049093140597No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTGTCCTTC1790.10170916859857267No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)