FastQCFastQC Report
Fri 18 Apr 2025
Yell_July18_Site1c_S85_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYell_July18_Site1c_S85_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128723
Total Bases38.7 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC2743621.313984291851497No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC1757513.653348663409027No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC1725013.40086853165324No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC53154.1290212316369255No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC35112.7275622849063494No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC32512.5255781795017205No Hit
TGATCCTTCTGCAGGTTCACCTACAGATACCTTGTTACGACTTAACCCTC14131.0977059266797697No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTAGTTC12520.9726311537176728No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTTTACTTC9990.776085081920092No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTGTCCTTC8450.6564483425650427No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTCCTTC8000.6214895550911648No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCACCTTC7300.5671092190206878No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCTCCTTC6980.5422496368170412No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4130.3208439828158138No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG3800.29520753866830324No Hit
TAGACCTCGGGGTGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG3350.26024875119442525No Hit
TGATCCTTCTGCAGGTTCACCTACGGCTACCTTGTTACGACTTCACCCCA2840.2206287920573635No Hit
TAGACTTCGGGGTGGCCGAAAAATCAAAATAAATGTTGGTATAACACTGG2570.19965351957303668No Hit
TAGACTTCGGGGTGGCCAAAGAATCAAAATAAATGTTGGTATAACACTGG2310.1794551090325738No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTTTAGTTC2230.17324021348166216No Hit
TAGACCTCGGGGTGGCCAAAGAATCAAAATAAATGTTGGTATAACACTGG2220.1724633515377982No Hit
TAGACTTCGGGATGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG2180.16935590376234239No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2150.16702531793075054No Hit
TAGACCTCGGGATGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG2090.1623641462675668No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCATC2070.1608104223798389No Hit
TAGACTTCGGGGTGGCCGAAGAACCAAAATAAATGTTGGTATAACACTGG1960.15226494099733537No Hit
TGATCCTTCTTCAGGTTCACCTACAGATACCTTGTTACGACTTAACCCTC1940.15071121710960744No Hit
TAGACCTCGGGGTGGCCGAAAAATCAAAATAAATGTTGGTATAACACTGG1940.15071121710960744No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTTTCCTTC1740.13517397823232835No Hit
TAGACTTCGGGATGGCCAAAGAATCAAAATAAATGTTGGTATAACACTGG1710.13284339240073648No Hit
TAGACTTCGGGATGGCCGAAAAATCAAAATAAATGTTGGTATAACACTGG1670.12973594462528065No Hit
TGATCCTTCTGCAGGTTCACCTACGGATACCTTGTTACGACTTTTACGTC1670.12973594462528065No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCTACTTC1630.1266284968498248No Hit
TAGACCTCGGGGTGGCCGAAGAACCAAAATAAATGTTGGTATAACACTGG1620.12585163490596085No Hit
TAGACTTCGGGGTGGCCAAAAAATCAAAATAAATGTTGGTATAACACTGG1600.12429791101823295No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTGTCCTTC1580.12274418713050504No Hit
TGATCCTTCTGCAGGTTCACCTACGGAGACCTTGTTACGACTTCTCCTTC1400.10876067214095383No Hit
TAGACCTCGGGATGGCCAAAGAATCAAAATAAATGTTGGTATAACACTGG1400.10876067214095383No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAAAAGGTGTTGGTAGAGTACTGG1390.10798381019708989No Hit
TAGACTTCGGGGTGGCCGAAAAACCAAAATAAATGTTGGTATAACACTGG1380.10720694825322591No Hit
TGATCCTTCTTCAGGTTCACCTACAGAAACCTTGTTACGACTTTTACTTC1320.10254577659004219No Hit
TAGACCTCGGGGTGGCCAAAAAATCAAAATAAATGTTGGTATAACACTGG1300.10099205270231426No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTGCTTC1290.10021519075845031No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)