FastQCFastQC Report
Fri 18 Apr 2025
Yell_July18_Site1bb_S139_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYell_July18_Site1bb_S139_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167944
Total Bases50.5 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC3428920.41692468918211No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC3408920.29783737436288No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC2836416.888962987662552No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC48552.890844567236698No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC47982.8569046825132185No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC42372.5228647644452913No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTGTCCTTC40602.417472490830277No Hit
TGATCCTTCTGCAGGTTCACCTACAGATACCTTGTTACGACTTAACCCTC39302.3400657361977806No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTAGTTC12020.715714762063545No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTCCTTC10920.6502167389129709No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCTCCTTC6740.40132425094078983No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCACCTTC6470.38524746344019434No Hit
TGATCCTTCTGCAGGTTCACCTACGGCTACCTTGTTACGACTTCACCCCA5850.3483303958462345No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTGTCCTTC5460.32510836945648547No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTTTACTTC5220.3108178916781784No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4190.2494879245462773No Hit
TGATCCTTCTTCAGGTTCACCTACAGATACCTTGTTACGACTTAACCCTC3950.23519744676797028No Hit
TGATCCTTCTGCAGGTTCACCTACGGAGACCTTGTTACGACTTCTCCTTC3910.23281570047158578No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCATC3460.20602105463726003No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCTGCTTC2900.17267660648787692No Hit
TGATCCTTCTGCAGGTTCACCTACGAAAACCTTGTTACGACTTCTCCTTC2040.12146906111560997No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)