FastQCFastQC Report
Fri 18 Apr 2025
Yell_July18_Site1b_S84_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYell_July18_Site1b_S84_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158246
Total Bases47.6 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC2687416.982419776803205No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC2577916.290459158525334No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC2242514.170974305827636No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC36652.31601430683872No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC33892.1416023153823796No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC30861.950128281283571No Hit
TGATCCTTCTGCAGGTTCACCTACAGATACCTTGTTACGACTTAACCCTC26881.6986211341834863No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTGTCCTTC24621.555805517990976No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTAGTTC11510.7273485585733604No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTCCTTC10690.6755305031406798No Hit
TGATCCTTCTGCAGGTTCACCTACGAAAACCTTGTTACGACTTCTCCTTC5920.374101083123744No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCTCCTTC5410.34187278035463775No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCACCTTC5400.3412408528493611No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5370.33934507033353134No Hit
TGATCCTTCTGCAGGTTCACCTACGGATACCTTGTTACGACTTTTACGTC5170.32670652022799945No Hit
TGATCCTTCTGCAGGTTCACCTACGGCTACCTTGTTACGACTTCACCCCA5070.32038724517523354No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTTTACTTC4590.290054724921957No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCATC3600.22749390189957408No Hit
TGATCCTTCTGCAGGTTCACCTACGAAAACCTTGTTACGACTTTTACTTC3550.22433426437319112No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTGTCCTTC3230.20411258420434006No Hit
TGATCCTTCTTCAGGTTCACCTACAGATACCTTGTTACGACTTAACCCTC2990.1889463240777018No Hit
TGATCCTTCTGCAGGTTCACCTACGGAGACCTTGTTACGACTTCTCCTTC2810.1775716289827231No Hit
TAGACTTCGGGGTGGCCGAAGAATCAGAAAAGATGTTGAAATAGGACGGG2400.1516626012663827No Hit
TAGACCTCGGGGTGGCCGAAGAATCAGAAAAGATGTTGAAATAGGACGGG2370.14976681875055292No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2280.14407947120306358No Hit
TAGACTTCGGGGTGGCCAAAGAATCAGAAAAGATGTTGAAATAGGACGGG1980.12512164604476575No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG1960.12385779103421254No Hit
TAGACTTCGGGGTGGCCGAAAAATCAGAAAAGATGTTGAAATAGGACGGG1940.12259393602365937No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAAAAGGTGCTGGAACAAAATAGG1780.11248309593923383No Hit
TAGACTTCGGGATGGCCGAAGAATCAGAAAAGATGTTGAAATAGGACGGG1780.11248309593923383No Hit
TAGACCTCGGGGTGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG1730.10932345841285088No Hit
TAGACCTCGGGGTGGCCGAAAAATCAGAAAAGATGTTGAAATAGGACGGG1650.10426803837063811No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)