FastQCFastQC Report
Fri 18 Apr 2025
Yell_July18_Site1a_S83_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYell_July18_Site1a_S83_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221836
Total Bases66.7 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC4703521.202600119006835No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC4526120.402910258028452No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC4293919.35619106006239No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC61062.7524838168737267No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC56252.535656971817018No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC53692.42025640563299No Hit
TGATCCTTCTGCAGGTTCACCTACAGATACCTTGTTACGACTTAACCCTC41481.869849798950576No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTGTCCTTC34621.560612344254314No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTAGTTC29411.3257541607313512No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTTTACTTC20840.9394327340918517No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCTCCTTC18300.8249337348311365No Hit
TGATCCTTCTGCAGGTTCACCTACAGAAACCTTGTTACGACTTCACCTTC16330.7361293928848338No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTCCTTC12320.555365224760634No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACGTCTCCTTC8970.40435276510575374No Hit
TGATCCTTCTGCAGGTTCACCTACGGCTACCTTGTTACGACTTCACCCCA8240.3714455724048396No Hit
TGATCCTTCTGCAGGTTCACCTACGGATACCTTGTTACGACTTTTACGTC6820.3074343208496367No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCATC6150.2772318289186606No Hit
TGATCCTTCTGCAGGTTCACCTACAGATACCTTGTGACGACTTAACCTAC6080.27407634468706615No Hit
TGATCCTTCTGCAGGTTCACCTACGGAGACCTTGTTACGACTTCTCCTTC5780.26055284083737534No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4400.19834472312879786No Hit
TGATCCTTCTGCAGGTTCACCTACGAAAACCTTGTTACGACTTTTACTTC4330.19518923889720333No Hit
TGATCCTTCTTCAGGTTCACCTACAGATACCTTGTTACGACTTAACCCTC4000.1803133846625435No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTGTCCTTC3870.17445319966101083No Hit
TGATCCTTCTGCAGGTTCACCTACGGTAACCTTGTTACGACTTCACCTTC3340.15056167619322383No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTTTAGTTC3190.14379992426837845No Hit
TGATCCTTCTGCAGGTTCACCTACGGTTACCTTGTTACGACTTCACCCCA3100.1397428731134712No Hit
TGATCCTTCTGCAGGTTCACCTACAGCAACCTTGTTACGACTTTTGCTTC2570.11585134964568419No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAGCCTTGTTACGACTTCTCCTTC2290.10322941271930616No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)