FastQCFastQC Report
Fri 18 Apr 2025
Yell_July18_Site1U_S114_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYell_July18_Site1U_S114_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42930
Total Bases12.9 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC3150.7337526205450734No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3100.7221057535522944No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAAAAGGTGTTGGTAGAGTACTGG2790.649895178197065No Hit
TAGACCTCGGGGTGGCCGAAGAATCAAAAAAGGTGTTGGTAGAGTACTGG2440.5683671092476124No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG2200.5124621476822734No Hit
TAGACTTCGGGGTGGCCGAAAAATCAAAAAAGGTGTTGGTAGAGTACTGG2150.5008152806894945No Hit
TAGACTTCGGGGTGGCCGAAGAACCAAAAAAGGTGTTGGTAGAGTACTGG1770.4122990915443746No Hit
TAGACCTCGGGGTGGCCGAAAAATCAAAAAAGGTGTTGGTAGAGTACTGG1750.407640344747263No Hit
TAGACTTCGGGGTGGCCGAAAAATCAAAATAAATGTTGGTATAACACTGG1650.38434661076170507No Hit
TAGACCTCGGGGTGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG1630.37968786396459353No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC1610.37502911716748194No Hit
TAGACCTCGGGGTGGCCGAAGAACCAAAAAAGGTGTTGGTAGAGTACTGG1610.37502911716748194No Hit
TAGACTTCGGGGTGGCCGAAGAACCAAAATAAATGTTGGTATAACACTGG1550.3610528767761472No Hit
TAGACTTCGGTGTGGCCGAAGAATCAAAAAAGGTGTTGGTAGAGTACTGG1410.32844164919636615No Hit
TAGACCTCGGTGTGGCCGAAGAATCAAAAAAGGTGTTGGTAGAGTACTGG1260.29350104821802936No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1250.2911716748194736No Hit
TAGACTTCGGGGTGGCCGAAAAACCAAAATAAATGTTGGTATAACACTGG1250.2911716748194736No Hit
TAGACTTCGGGGTGGCCAAAGAATCAAAATAAATGTTGGTATAACACTGG1150.2678779408339157No Hit
TAGACTTCGGGGTGGCCAAAGAATCAAAAAAGGTGTTGGTAGAGTACTGG1130.2632191940368041No Hit
TAGACCTCGGGATGGCCGAAGAATCAAAAAAGGTGTTGGTAGAGTACTGG1120.26088982063824834No Hit
TAGACCTCGGGGTGGCCGAAGAACCAAAATAAATGTTGGTATAACACTGG1110.2585604472396925No Hit
TAGACCTCGGGGTGGCCGAAAAACCAAAAAAGGTGTTGGTAGAGTACTGG1110.2585604472396925No Hit
TAGACTTCGGGGTGGCCGAAAAACCAAAAAAGGTGTTGGTAGAGTACTGG1100.2562310738411367No Hit
TAGACTTCGGGGTGGCCGAAGAATCAGAAAAGATGCTGGTAAAGCACTGG1080.25157232704402516No Hit
TAGACCTCGGGGTGGCCGAAGAATCAGAAAAGATGCTGGTAAAGCACTGG1060.24691358024691357No Hit
TAGACCTCGGGGTGGCCGAAAAATCAAAATAAATGTTGGTATAACACTGG1060.24691358024691357No Hit
TAGACTTCGGTGTGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG1050.2445842068483578No Hit
TAGACTTCGGTGTGGCCGAAAAATCAAAAAAGGTGTTGGTAGAGTACTGG980.22827859305846726No Hit
TAGACTTCGGGATGGCCGAAGAATCAAAAAAGGTGTTGGTAGAGTACTGG960.22361984626135567No Hit
TAGACCTCGGGGTGGCCAAAGAATCAAAAAAGGTGTTGGTAGAGTACTGG950.2212904728627999No Hit
TAGACTTCGGTGTGGCCGAAGAACCAAAAAAGGTGTTGGTAGAGTACTGG940.2189610994642441No Hit
TAGACTTCGGGGTGGCCAAAAAATCAAAAAAGGTGTTGGTAGAGTACTGG840.1956673654786862No Hit
TAGACCTCGGGGTGGCCAAAAAATCAAAAAAGGTGTTGGTAGAGTACTGG830.19333799208013044No Hit
TAGACCTCGGGGTGGCCAAAGAATCAAAATAAATGTTGGTATAACACTGG830.19333799208013044No Hit
TAGACTTCGGGGTGGCCAAAGAACCAAAATAAATGTTGGTATAACACTGG820.19100861868157465No Hit
TAGACCTCGGGATGGCCGAAGAACCAAAAAAGGTGTTGGTAGAGTACTGG810.18867924528301888No Hit
TAGACCTCGGTGTGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG810.18867924528301888No Hit
TAGACCTCGGTGTGGCCGAAGAACCAAAAAAGGTGTTGGTAGAGTACTGG800.1863498718844631No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTCACCTTC800.1863498718844631No Hit
TAGACTTCGGGGTGGCCGAAGAATCAAAAAATATGCTGAAACAGGACAGG780.1816911250873515No Hit
TAGACTTCGGGGTGGCCAAAAAATCAAAATAAATGTTGGTATAACACTGG780.1816911250873515No Hit
TAGACTTCGGGGTGGCCAAAGAACCAAAAAAGGTGTTGGTAGAGTACTGG750.17470300489168414No Hit
TAGACCTCGGGGTGGCCGAAAAACCAAAATAAATGTTGGTATAACACTGG740.17237363149312834No Hit
TGATCCTTCTGCAGGTTCACCTACGGAAACCTTGTTACGACTTCTCCTTC740.17237363149312834No Hit
TAGACTTCGGTGTGGCCGAAAAATCAAAATAAATGTTGGTATAACACTGG720.16771488469601675No Hit
TAGACCTCGGTGTGGCCGAAAAATCAAAAAAGGTGTTGGTAGAGTACTGG680.15839739110179363No Hit
TAGACCTCGGGGTGGCCAAAGAACCAAAAAAGGTGTTGGTAGAGTACTGG670.15606801770323783No Hit
TAGACTTCGGGATGGCCGAAAAATCAAAAAAGGTGTTGGTAGAGTACTGG630.14675052410901468No Hit
TAGACTTCGGTGTGGCCGAAAAACCAAAAAAGGTGTTGGTAGAGTACTGG630.14675052410901468No Hit
TAGACTTCGGGGTGGCCGAAAAATCAGAAAAGATGCTGGTAAAGCACTGG630.14675052410901468No Hit
TAGACTTCGGTGTGGCCAAAGAATCAAAATAAATGTTGGTATAACACTGG620.14442115071045888No Hit
TAGACTTCGGGATGGCCGAAGAACCAAAAAAGGTGTTGGTAGAGTACTGG610.1420917773119031No Hit
TAGACCTCGGGGTGGCCAAAAAATCAAAATAAATGTTGGTATAACACTGG610.1420917773119031No Hit
TAGACTTCGGTGTGGCCAAAGAATCAAAAAAGGTGTTGGTAGAGTACTGG610.1420917773119031No Hit
TAGACCTCGGGGTGGCCGAAGAACCAGAAAAGATGCTGGTAAAGCACTGG600.1397624039133473No Hit
TAGACTTCGGGATGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG600.1397624039133473No Hit
TAGACTTCGGGGTGGCCGAAAAATCAAAAAATATGCTGAAACAGGACAGG590.13743303051479153No Hit
TAGACTTCGGTGTGGCCAAAAAATCAAAAAAGGTGTTGGTAGAGTACTGG590.13743303051479153No Hit
TAGACCTCGGGATGGCCGAAAAATCAAAAAAGGTGTTGGTAGAGTACTGG580.13510365711623573No Hit
TAGACCTCGGTGTGGCCAAAGAATCAAAAAAGGTGTTGGTAGAGTACTGG570.13277428371767994No Hit
TAGACCTCGGGATGGCCGAAAAACCAAAAAAGGTGTTGGTAGAGTACTGG560.13044491031912417No Hit
TAGACTTCGGGATGGCCAAAGAATCAAAAAAGGTGTTGGTAGAGTACTGG560.13044491031912417No Hit
TAGACCTCGGGGTGGCCGAAAAATCAGAAAAGATGCTGGTAAAGCACTGG560.13044491031912417No Hit
TAGACCTCGGGATGGCCGAAGAATCAAAATAAATGTTGGTATAACACTGG550.12811553692056835No Hit
TAGACCTCGGGGTGGCCAAAGAACCAAAATAAATGTTGGTATAACACTGG510.11879804332634522No Hit
TAGACTTCGGGGTGGCCGAAGAACCAGAAAAGATGCTGGTAAAGCACTGG510.11879804332634522No Hit
TAGACTTCGGTGTGGCCGAAGAACCAAAATAAATGTTGGTATAACACTGG510.11879804332634522No Hit
TAGACCTCGGTGTGGCCAAAGAACCAAAAAAGGTGTTGGTAGAGTACTGG500.11646866992778944No Hit
TAGACCTCGGTGTGGCCGAAAAACCAAAAAAGGTGTTGGTAGAGTACTGG490.11413929652923363No Hit
TAGACTTCGGGGTGGCCGAAAAACCAGAAAAGATGCTGGTAAAGCACTGG470.10948054973212205No Hit
TAGACCTCGGTGTGGCCGAAAAATCAAAATAAATGTTGGTATAACACTGG460.10715117633356627No Hit
TAGACCTCGGTGTGGCCGAAGAATCAGAAAAGATGCTGGTAAAGCACTGG460.10715117633356627No Hit
TAGACTTCGGGGTGGCCAAAAAACCAAAAAAGGTGTTGGTAGAGTACTGG460.10715117633356627No Hit
TAGACTTCGGTGTGGCCAAAGAACCAAAAAAGGTGTTGGTAGAGTACTGG460.10715117633356627No Hit
TAGACTTCGGGATGGCCAAAAAATCAAAAAAGGTGTTGGTAGAGTACTGG450.10482180293501049No Hit
TAGACCTCGGGGTGGCCGAAAAACCAGAAAAGATGCTGGTAAAGCACTGG450.10482180293501049No Hit
TAGACCTCGGGGTGGCCGAAGAATCAGAAAAGATGTTGGTAGAGTACTGG450.10482180293501049No Hit
TAGACCTCGGGGTGGCCGAAGAATCAGAAGAGATGTTGGTAGAGTACTGG450.10482180293501049No Hit
TAGACCTCGGGGTGGCCGAAGAATCAAAAAATATGCTGAAACAGGACAGG440.10249242953645471No Hit
TAGACTTCGGGGTGGCCAAAGAATCAGAAAAGATGCTGGTAAAGCACTGG440.10249242953645471No Hit
TAGACTTCGGTGTGGCCGAAAAATCAGAAAAGATGCTGGTAAAGCACTGG440.10249242953645471No Hit
TGATCCTTCTTCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTC440.10249242953645471No Hit
TAGACTTCGGGATGGCCGAAAAACCAAAAAAGGTGTTGGTAGAGTACTGG430.1001630561378989No Hit
TAGACTTCGGGGTGGCCGAAGAATCAGAAGAGATGTTGGTAGAGTACTGG430.1001630561378989No Hit
TAGACTTCGGGGTGGCCAAAAAACCAAAATAAATGTTGGTATAACACTGG430.1001630561378989No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)