FastQCFastQC Report
Fri 18 Apr 2025
Yell_July18_Site1J_S99_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYell_July18_Site1J_S99_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8723
Total Bases2.6 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACACACCGCCCGTCGCTACTACCGATTGAATGGTTTAGTGAGATCTTC1121.2839619396996447No Hit
GTACACACCGCCCGTCGCACCTACCGATTGAATGGTCCGGTGAGGCCTCG500.5731972945087699No Hit
GTACACACCGCCCGTCGCTACTACCGATTGAATGGTTTAGTGAGATGTTC320.36684626848561275No Hit
GTACACACCGCCCGTCGCACCTACCGATTGAATGATTCGGTGAAAATCTC260.29806259314456035No Hit
GTACACACCGCCCGTCGCTACTACCGATTGAACATTTTAGTGAGGTCCTC260.29806259314456035No Hit
GGCGTGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG250.28659864725438494No Hit
GTACACACCGCCCGTCGCTCCTACCGATTGAGTGATCCGGTGAATAATTC240.2751347013642096No Hit
GGCGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG220.25220680958385877No Hit
GTACACACCGCCCGTCGCTCCTACCGATTTCGAGTGGTCCGGTGAATCTT190.21781497191333257No Hit
GTACACACCGCCCGTCGCTACTACCGATTGGATGGTTTAGTGAGATCCTC170.19488708013298178No Hit
GGCGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG150.17195918835263097No Hit
GGGGTGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG140.16049524246245558No Hit
GTACACACCGCCCGTCGCTACTACCGATTGAATGTCATAGTGAGGTCCTC110.12610340479192939No Hit
GGAACTGGTTGAACAGTTTATCCCCCTTTATCTGGACCACAAATGCACTC100.11463945890175398No Hit
GTACACACCGCCCGTCGCTACTACCGATTGGACGTTTTAGTGAGACATTT100.11463945890175398No Hit
GGAACTGGTTGAACAGTTTACCCTCCTTTATCTGGACCACAAATGCACTC90.10317551301157858No Hit
GGTACAGGTTGAACTGTATATCCTCCTTTATCTGGACCACAAATGCACTC90.10317551301157858No Hit
GGAACTGGTTGAACAGTTTACCCCCCTTTATCTGGACCACAAATGCACTC90.10317551301157858No Hit
GGAACTGGTTGAACAGTTTATCCTCCATTATCTAGTGGTAATTCTCATTC90.10317551301157858No Hit
GGTACTGGTTGAACAGTTTATCCTCCATTATCTAGTGGTAATTCTCATTC90.10317551301157858No Hit
GTACACACCGCCCGTCGCACCTACCGATTGAATGGTCCGGTGAAGCCTCG90.10317551301157858No Hit
GGAACTGGTTGAACTGTATATCCTCCTTTATCTGGACCACAAATGCACTC90.10317551301157858No Hit
GGAACAGGATGAACTGTTTACCCTCCTTTATCTGGACCACAAATGCACTC90.10317551301157858No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)