FastQCFastQC Report
Mon 10 Jun 2024
150.flexbar_trim.R_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename150.flexbar_trim.R_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25502427
Total Bases3.4 Gbp
Sequences flagged as poor quality0
Sequence length18-150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4651171.8238146510526232No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA462710.18143763336720853No Hit
CTGTATGCCAGCATAGGCAGTGACCAGCGGCGCTTAGTGAAAACACACAC373030.14627235282351755No Hit
GTTTTTTTGGTTTGTGTGCCTTTTGCATCATGCTATTTGGATAGTTTATA300830.11796132187732564No Hit
CCCACACATACACACACACACACACACACACACACACACACACACACACA257020.10078256473393689No Hit
ACTGTATGCCAGCATAGGCAGTGACCAGCGGCGCTTAGTGAAAACACACA255440.10016301585727508No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)