FastQCFastQC Report
Thu 6 Jun 2024
80_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename80_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14758655
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTCAT444300.30104369266711634No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGACTATCTGGGGG223900.1517075912405297TruSeq Adapter, Index 10 (97% over 37bp)
GTTTTTTCTTTATTTTTCCTCACACACAGACACTCACTCAAACACAAACC211100.14303471420668076No Hit
GTGAGATCGAAAGCTATTGAGTCCCACCATTCTGTTGCAGAGAAAGTGCA205580.13929453598583338No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGACTATCTAGGGG172920.11716514817915319TruSeq Adapter, Index 10 (97% over 37bp)
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG170710.1156677217537777No Hit
CTCACACACAGACACTCACTCAAACACAAACCTTAATCAGTGCATCAAAC156700.10617498681282271No Hit
TTTTTCTTTATTTTTCCTCACACACAGACACTCACTCAAACACAAACCTT155100.10509087718359159No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)