FastQCFastQC Report
Thu 6 Jun 2024
37_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename37_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16337076
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATCTGAATCTGGGGG908710.556225606099892TruSeq Adapter, Index 2 (97% over 37bp)
GAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTCAT217580.1331817272564564No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185260.1133985053384094No Hit
GTCCATTTCATAATCCGGAAAATCTGTTATACCAAAAATGAAGCCATAGC163610.10014643991372751No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)