FastQCFastQC Report
Thu 6 Jun 2024
2_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19610239
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1551850.7913468061251063No Hit
GGAAAATTCGCTTTGGCACTCATCCTCTGCCTCCAGGTCTCCCTGAGCCT349710.17833030999775168No Hit
TGTCACTTCTTGGTTCTGCACGTCTAACTCACCAGCAGAGATGAACGGAA343400.17511260316613175No Hit
CTCCACCAGACCAACACCATGAAATTCGCCGCTCTTGCTCTTGCCCTTCT238330.12153344994928414No Hit
TGAAGGCCTACCTGCTGTTACTGCTCCTGCTCCCGGTCTGCCTGGCTGAC209640.10690333758808346No Hit
CTGTATGCCAGCATAGGCAGTGACCAGCGGCGCTTAGTGAAAACACACAC196140.1000191787565669No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)