FastQCFastQC Report
Thu 6 Jun 2024
139_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename139_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9694605
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATGAGATCTGGGGG522620.5390833355252741TruSeq Adapter, Index 7 (97% over 38bp)
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG199850.20614558303303746No Hit
GAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTCAT135890.1401707444501349No Hit
GTGAGATCGAAAGCTATTGAGTCCCACCATTCTGTTGCAGAGAAAGTGCA130980.1351060718822479No Hit
CTCACACACAGACACTCACTCAAACACAAACCTTAATCAGTGCATCAAAC105370.10868931740901254No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)