FastQCFastQC Report
Thu 6 Jun 2024
138_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename138_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8479903
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATGAGATCTGGGGG751080.8857176786102388TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATGAGATCTGGGGT113960.13438832967782768TruSeq Adapter, Index 7 (97% over 38bp)
CAAAAATGAAGCCATAGCAGTAATCAATAATACCTTCTAGAGTCTGACTA106910.12607455533394663No Hit
GTCGTATGCAAGTGGTTTAACACCCAGTCCTTCATAAACATGTGGTGTGA96670.11399894550680591No Hit
TTTTGTTATTAACATCACCAAGTCAACCCAACGAAATTAACCGGATACAA85560.10089738054786712No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)