FastQCFastQC Report
Thu 5 Dec 2024
88-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename88-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18003518
Total Bases512.9 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGACTAGGAGATTTGT2959411.6437953959887175No Hit
TATCGAACTATTGATGAACTTTAACATCTT1697720.9429934749419531No Hit
TGATCTGAACTTTTTATCACATTGCAACCC1043280.5794867425355422No Hit
TATCACAGCTGGCTTGAGTGAGC1009260.560590435713731No Hit
TTTTACATCTTTGTGACGTCTGCCAGAGC590620.32805810508812777No Hit
TGGACGGAGAACTGATAAGGGT548390.304601578424839No Hit
TAAGGTCTGAAGCTCCTCATTACAAGAGCAC520900.28933234049034195No Hit
TCTATGTCTCTTGTGTTCTTTATGAAGATT507190.2817171621679718No Hit
TATGTCCGGGCTATAGAGAGAACCTCTTC446530.2480237473587107No Hit
TGATCTGAACTTTTTATCACATTGCAACCT383150.21281951671889904No Hit
TTCGACTAGGAGATTTGC348260.19343997101011037No Hit
TATGTCTGTTCATGCATCCCTCTGTATGTC314840.17487693238621474No Hit
TATGTCCGGGCTATAGAGAGAACCTCTTCAT280670.1558973085149247No Hit
TAAGACTGTATCTGAAGCTATGTTGGGCAT259210.14397741596947886No Hit
TACGTGAAATCGCTCAGGACTTCAAGACTG252530.14026703003268584No Hit
TACCTTTGTGACTCTGTACAGGTCCCTGTC242990.13496806568582875No Hit
TCACTGGTCTGGTGGCGTGTAATGGCCTTC235450.13077999533202345No Hit
TCGTAGTCTGGATGGCCACATGGACGTTC220380.12240940909437811No Hit
TGTGGTCTTTTGTGGAAAATTATTTTCCTGA219200.12175398163847755No Hit
TTTTCCATGTTCTCTCTGATATTTGAAGTT214430.119104499465049No Hit
TTATTACACTCAAAATATTCAAGACTGATG210640.11699935534821583No Hit
CTTTCGACTAGGAGATTTGT207280.1151330534398888No Hit
TGATCTGAACTTTTTATCACATTTAAGCC206720.1148220031218343No Hit
TATCACAGCTGGCTTGAGTGAGT197550.10972855416369177No Hit
TGATTATGGACTACACGAATATGCAACTGT195780.10874541297984094No Hit
AATTGCACTTGTCCCGGCCTGC193590.1075289840574492No Hit
TCAGACTGTCTGTCAGAAAATAAAGCAGC190430.10577377154842736No Hit
ATTGGTCGAACTGGTTATCAGCTTTT180890.10047480720157026No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)