FastQCFastQC Report
Thu 5 Dec 2024
71-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename71-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18880938
Total Bases516.2 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCCGTAGATCCGAACTTGTG2903221.5376460639826264No Hit
AACCCGTAGATCCGAACTTGT2710691.4356754945119783No Hit
AATTGCACTTGTCCCGGCCTGC1408980.7462447045798255No Hit
TCTAAATGGATCTGCTGCAATAATCGAGGC1248720.6613654469920933No Hit
TGTAGTTTGGTATCAGAATTATTAGACGT995770.5273943487341571No Hit
TGGAAGACTTGTGATTTTGTTGT899820.4765758989304451No Hit
AACCCGTAGATCCGAACTTGTGA859520.45523162037818243No Hit
TGTAGATCTGAACATTTCATATTGTAGTTC848790.4495486400093046No Hit
ATGTGGTCCGGAGTTAGCTGGTTATC795940.42155744592773936No Hit
TATCGAACTATTGATGAACTTTAACATCTT788350.41753751852794607No Hit
TGAATTATGGATTTGGAACATA616110.32631323719192346No Hit
TTCGACTCACCTGGGAGCTTCC587830.3113351677760925No Hit
TGCCGAATTGTAGTCTATAGAAGC575540.3048259572697077No Hit
CTTGCCGTATGTGTCCCTTGACCGCCAC505550.2677568243696367No Hit
TGTAGTTTGGTGTCAGAATTATTAGACGT413720.21912046954446862No Hit
AACCCGTAGATCCGAACTTGC369110.19549346541999132No Hit
TAGTAAATCTGTGTCTGCTAATCTCTTGC360610.19099157043998555No Hit
AACCCGTAGATCCGAACTTGTA354560.1877872804836285No Hit
ATGTGGTCCGGAGTTAGCTGGTTAT351030.1859176699801673No Hit
TGAGGTAGTAGGTTGTATAGT325070.17216835307652617No Hit
AATTGCACTTGTCCCGGCCTGT323180.1711673434868543No Hit
TCTAAATGGATCTGCTGCAATAATCGAGGT304820.16144325033004187No Hit
TGAACGAACTCTGAAATACACACAATCATG294870.15617338502991746No Hit
TCTGATCTCTTGGCTGTTTTTAGAGCTTG281100.14888031516230815No Hit
ATGTGGTCCGGAGTTAGCTGGTTATCTATG279030.14778397132600088No Hit
TGAAAGACATGGGTAGTGAGAT260200.1378109498585293No Hit
TCTAAATGGATCTGCTGCAACAATCGAGGC251590.1332507950611352No Hit
TACGTGAAATCGCTCAGGACTTCAAGACTG248640.13168837268572145No Hit
TCTATGTCTCTTGTGTTCTTTATGAAGATT224740.1190301032713523No Hit
TTCGACTCACCTGGGAGCTTCCA217850.11538092016403No Hit
TATCGAACTATTGATGAATCTTAACATCTT211780.11216603751360234No Hit
TGAAAGACATGGGTAGTGAGATG208150.11024346353978812No Hit
TCACAACCTGCTTGAATGAGGAC205580.10888230235171578No Hit
TGATCTGAACTTTTTATCACATTTAAGCC195990.10380310554486223No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)