FastQCFastQC Report
Thu 5 Dec 2024
501-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename501-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17161695
Total Bases486.8 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGACTAGGAGATTTGT3573612.0823176265514567No Hit
TATCACAGCTGGCTTGAGTGAGC1169030.6811856288088094No Hit
TGTAGTTCTTCCAGATGTCTCAGATATTG964150.5618034815325642No Hit
TGGACGGAGAACTGATAAGGGT708940.4130943942308729No Hit
TATCGAACTATTGATGAACTTTAACATCTT467630.2724847399980014No Hit
TCGACTAGGAGATTTGT406060.23660833035431522No Hit
TGATCTGAACTTTTTATCACATTGCAACCC385200.22445335382082016No Hit
TTCGACTAGGAGATTTGC384050.22378325684030628No Hit
TGTGATCTTCTGGGAAATAGATAATTCCGT338050.19697937761975143No Hit
TCAGATCTGAATAACTCAATATGAACAAGTC336580.19612281887074673No Hit
TGTATCGGTCTTTTGGTGCTTACACCTGG334900.19514389458616996No Hit
TAAGATCTCTAGATTATCCATTTTGTCTG325600.18972484943940562No Hit
TATCACAGCTGGCTTGAGTGAGT317580.1850516513666045No Hit
TATCGAACTATTGATGAATCTTAACATCTT291300.16973847862929622No Hit
TTGACGTAATTGACTTTGACCTATATTTTT243010.14160023237797897No Hit
TGTAGAAATCTCTTGATCATCTTGGTTTTG218050.1270562144356953No Hit
TGAATACTCGGTCTAAAGCTTCAACAATTT216000.12586169373130102No Hit
TACGAATATCCGGATCTGAAAACTGAATTC213280.12427676869912907No Hit
TACGTGAAATCGCTCAGGACTTCAAGACTG209370.12199843896538191No Hit
TCCGAGAAACTGTTGTACACATTGTATAC201060.11715625991488603No Hit
TATCGAACTATTGATAAACTTTAATATCTT198800.11583937367491963No Hit
TGCTTGTGGATTTTTGTCACCATCTGATA195900.11414956389797161No Hit
CGAGAACTCTTTGGAGAAAATCCTCCTCAG190770.1111603486718532No Hit
TAAGACTGTATCTGAAGCTATGTTGGGCAT189340.11032709764390988No Hit
ATTGGTCGAACTGGTTATCAGCTTTT186430.10863146093669653No Hit
TTACTATCCGATTTTGACTTAAAGCTCA183470.10690668957815647No Hit
AATTGCACTTGTCCCGGCCTGC178000.10371935872301657No Hit
TCGGGAATATCACTCTCTACTGAGTCAAGT172670.10061360489159142No Hit
TGATCTGAACTTTTTATCACATTGCAACCT172320.10040966233230458No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)