FastQCFastQC Report
Thu 5 Dec 2024
485-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename485-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15645635
Total Bases442.3 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGACTAGGAGATTTGT4593052.9356750301282113No Hit
TATCGAACTATTGATGAACTTTAACATCTT1715891.0967212260799897No Hit
TATCACAGCTGGCTTGAGTGAGC1104050.7056600770758106No Hit
TCGACTAGGAGATTTGT594870.38021467329386116No Hit
TGATCTGAACTTTTTATCACATTGCAACCC529070.338158214735292No Hit
TGGACGGAGAACTGATAAGGGT514210.3286603579848309No Hit
TTCGACTAGGAGATTTGC511380.32685154677326933No Hit
TACGTTGTGTTTCTTTCGGTTGTAAAAGC480710.3072486351624591No Hit
TATTTGGTCTGCAAAGTTGTCTTTATAGC352300.22517462538273456No Hit
TACGTGAAATCGCTCAGGACTTCAAGACTG348630.22282892321085082No Hit
TGTAGTTCTTCCAGATGTCTCAGATATTG335730.21458381203447477No Hit
TCGACTAGGAGATTTGTT308700.19730742791839387No Hit
TGAATACTCGGTCTAAAGCTTCAACAATTT282320.18044649514065744No Hit
TATCACAGCTGGCTTGAGTGAGT271050.17324320808966848No Hit
TGGCTATATCATCAGGATCTGTGAATAT261950.16742688935284508No Hit
TAAGACTGTATCTGAAGCTATGTTGGGCAT223050.14256372464268788No Hit
TGATCTGAACTTTTTATCACATTGCAACCT221680.14168808105263864No Hit
TGATTTAATTTGTGACTGTTTTGCTGATT196580.1256452678334884No Hit
TTTTACAGTAGAATATTCTA191090.12213630191423998No Hit
TGAACGAACTCTGAAATACACACAATCATG188230.12030831602552405No Hit
TCATCTTCAGTATATCTAACTTTGAACAAGT183520.11729789171228908No Hit
CAACTGTTGATTTCTGAGGGCTAACCAAAG177800.11364191993485723No Hit
CAACTCTATGCGGTGGATCACTCGGCTCGTG177550.11348213095857086No Hit
TTTTACATCTTTGTGACGTCTGCCAGAGC175330.11206320484914803No Hit
AATTGCACTTGTCCCGGCCTGC170330.10886742532342088No Hit
TCCGAGAAACTGTTGTACACATTGTATAC167300.10693078293083022No Hit
TATCACAGCCTGCTTTGATGAGC165350.10568442891579664No Hit
TATCGAACTATTGATGAACTTTAACATCT164250.10498135742013667No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)