FastQCFastQC Report
Thu 5 Dec 2024
460-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename460-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14767871
Total Bases416.3 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGACTAGGAGATTTGT4304252.9146042784366144No Hit
TATCACAGCTGGCTTGAGTGAGC989610.6701101330042767No Hit
TGGACGGAGAACTGATAAGGGT486210.329234999411899No Hit
TTCGACTAGGAGATTTGC454740.307925224969801No Hit
TCGACTAGGAGATTTGT431670.2923034742110085No Hit
TAAGGTCTGAAGCTCCTCATTACAAGAGCAC392550.26581353534304303No Hit
TGCCGAATTGTAGTCTATAGAAGC364320.24669771289307715No Hit
TATCACAGCTGGCTTGAGTGAGT241730.1636864244006465No Hit
TGAAGACGTTGAAGATCTTGATGCGATTG235620.15954906431671836No Hit
TATCACAGCCTGCTTTGATGAGC234160.15856043162890573No Hit
TAAGATCTCTAGATTATCCATTTTGTCTG230150.15584507746580398No Hit
ATTGGTCGAACTGGTTATCAGCTTTT225890.1529604368835562No Hit
TCCGAGAAACTGTCGTACACATTGTATAC224070.15172803175217334No Hit
TTTGTCTCGGTTCGCCATCTGGATGGCATG218590.1480172734444931No Hit
TCTAGTCTTTCCAGTGTAGAAGCTGAGGTT194360.13161003370086316No Hit
TACGTGAAATCGCTCAGGACTTCAAGACTG183510.12426300310992694No Hit
TGTATTCTCAATGTAGTTTATCTAGGCGT172460.11678054338367391No Hit
CATGATGTATTGCCTGTAGCCTTGGTGAT172260.11664511424835712No Hit
TGTGCTTTTGAACCTGTTCTAAAAATGTTC168500.11409904650440134No Hit
TAAGACTGTATCTGAAGCTATGTTGGGCAT165930.11235878211558051No Hit
CTTTCGACTAGGAGATTTGT165200.11186446577167419No Hit
TGATATCCTCCTGAATGTCGTGTAGCTGG160380.10860062361053939No Hit
TGATCTGAACTTTTTATCACATTGCAACCC159740.10816725037752564No Hit
TCCGGTTTGTGAAATGAGCGTCTCCTTTGC159660.10811307872339891No Hit
TGTAGTTTGGTATCAGAATTATTAGACGT154620.10470026451341564No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)