FastQCFastQC Report
Thu 5 Dec 2024
376-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename376-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29730313
Total Bases842.7 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGACTAGGAGATTTGT7835842.635639927504295No Hit
TGTAGTTTGGTATCAGAATTATTAGACGT2117440.7122158451544052No Hit
TATCACAGCTGGCTTGAGTGAGC1534720.5162138723531098No Hit
TCCTTCATGATTTAAGCACTGTAAAAATT1410460.47441814689270173No Hit
TCGACTAGGAGATTTGT951520.32005044817388906No Hit
TCCGAGAAACTGTTGTACACATTGTATAC909090.3057788190793686No Hit
TTCGACTAGGAGATTTGC843410.28368688886659216No Hit
TGTATCGGTCTTTTGGTGCTTACACCTGG820630.27602467555588805No Hit
TGGACGGAGAACTGATAAGGGT776140.2610601509644382No Hit
TGTGATCTTCTGGGAAATAGATAATTCCGT674900.22700736450369696No Hit
TCTATGTCTCTTGTGTTCTTTATGAAGATT615400.20699412078170856No Hit
TTTGTGCTCTGTTTTGACCTCAATGAAGGC577880.1943740047405488No Hit
TGACTAAACTCTTCCAGTTTTCATGATGG555170.1867353364224588No Hit
TTCAATAATTTTATGACTTCCTGACGTGT539430.18144107665465883No Hit
TAAGATCTCTAGATTATCCATTTTGTCTG498040.16751925887897648No Hit
TTAGATCTCAAGTAGTATTTGTTTACAGG496620.1670416318859475No Hit
TGAATACTCGGTCTAAAGCTTCAACAATTT463640.1559485767943311No Hit
TATCGAACTATTGATGAACTTTAACATCTT405060.13624478154669947No Hit
TAACTAATAGCAAACTCTTCACGATCAAAG400230.1346201770563263No Hit
TGAAGACGTTGAAGATCTTGATGCGATTG386150.12988426997051797No Hit
TATCACAGCTGGCTTGAGTGAGT383580.129019832384543No Hit
CAACTGTTGATTTCTGAGGGCTAACCAAAG370900.12475482515101673No Hit
TAACTAATAGCAAACTCTTCACGATCAAAGA366690.12333876202379707No Hit
CAACTCTATGCGGTGGATCACTCGGCTCGTG366530.12328494489782196No Hit
TACGATCTGTTTTGGCACTGTTTGCTCAGT364600.12263577581574739No Hit
TAAGACTGTATCTGAAGCTATGTTGGGCAT361300.12152579759251105No Hit
TTTGTCTCGGTTCGCCATCTGGATGGCATG358540.12059745216944068No Hit
TATTTGTAAAATCTGGCAATAAGAGACC358360.12053690790271869No Hit
TTCAGATCTTTGCCTGTTATACAGACTTTC354470.1192284790274492No Hit
TGATATCCTCCTGAATGTCGTGTAGCTGG349250.11747269529251171No Hit
TCCAGCTTCGTATCTGTTTATAAATTCAGC344670.11593218006147463No Hit
TGATCTGAACTTTTTATCACATTGCAACCC318650.10718016994977483No Hit
TGCTTTTCCGAATTCAGACTGTCAAATGA311210.10467767359193293No Hit
TGTATCGGTCTTTTGGTGCTTACACCTGGT308560.10378632744297042No Hit
TTTGTGCTCTGTTTTGACCTCAATGAAGG303150.10196663587093752No Hit
TGAATACACGATCTCTGGAGTCATTTAACG302490.10174464022629025No Hit
AATTGCACTTGTCCCGGCCTGC300710.10114592469981731No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)