FastQCFastQC Report
Thu 5 Dec 2024
367-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename367-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15680714
Total Bases445.3 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGACTAGGAGATTTGT3755742.3951332828339322No Hit
TGATCTGAACTTTTTATCACATTGCAACCC1440430.9185997525367787No Hit
TATCACAGCTGGCTTGAGTGAGC852500.5436614684764992No Hit
TGATCTGAACTTTTTATCACATTGCAACCT589690.3760606819306825No Hit
TGATCTGAACTTTTTATCACATTTAAGCC556840.35511138077003385No Hit
TCGACTAGGAGATTTGT499300.31841662312060537No Hit
TGTATCGGTCTTTTGGTGCTTACACCTGG462160.29473147715084913No Hit
TATCGAACTATTGATGAACTTTAACATCTT437870.27924111108716093No Hit
TTCGACTAGGAGATTTGC420620.26824033650508516No Hit
TGGACGGAGAACTGATAAGGGT420230.2679916233406209No Hit
TGTAGTTCTTCCAGATGTCTCAGATATTG409710.2612827451607114No Hit
TATTTGGTCTGCAAAGTTGTCTTTATAGC359020.2289564110409768No Hit
TCCGATCGTTAAGACAATGATGTCCAAAGC355940.2269922147677714No Hit
TGAATACTCGGTCTAAAGCTTCAACAATTT291870.18613310592872237No Hit
TAAGATCTCTAGATTATCCATTTTGTCTG291370.18581424289735785No Hit
TGATCTGAACTTTTTATTACATTGCAACCC264700.1688060888043746No Hit
CAACTCTATGCGGTGGATCACTCGGCTCGTG236520.15083496835667048No Hit
TACGTGAAATCGCTCAGGACTTCAAGACTG232640.14836059123328185No Hit
TCCGAGAAACTGTTGTACACATTGTATAC211490.13487268500656283No Hit
TATCACAGCTGGCTTGAGTGAGT205340.1309506697207793No Hit
TGATCTGAACTTTTTATCACATTGCAACC201700.12862934685244562No Hit
TGTATCGGTCTTTTGGTGCTTACACCTGGT199130.126990390871232No Hit
TGATCTGAACTTTTTATCACATTTAAGCT194250.12387828768511434No Hit
TCCGACTCTGTACATCATCTTTATTAGTGC187150.11935043263973821No Hit
TAACGCTCAGATCTGAATAACTCAATATG182400.11632123384177531No Hit
TCACTGGTCTGGTGGCGTGTAATGGCCTTC181690.1158684483372377No Hit
TGATATCCTCCTGAATGTCGTGTAGCTGG170630.10881519808345463No Hit
AATTGCACTTGTCCCGGCCTGC166200.10599007162556502No Hit
TGTGATCTTCTGGGAAATAGATAATTCCGT166170.10597093984368314No Hit
TCTAGTCTTTCCAGTGTAGAAGCTGAGGTT161700.10312030434328437No Hit
ATTGGTCGAACTGGTTATCAGCTTTT159430.10167266618088946No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)