FastQCFastQC Report
Thu 5 Dec 2024
363-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename363-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17268502
Total Bases492.8 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGACTAGGAGATTTGT3533112.0459852279022233No Hit
TGATCTGAACTTTTTATCACATTGCAACCC2629801.5228883200175674No Hit
TGATCTGAACTTTTTATCACATTTAAGCC1284470.7438224809540515No Hit
TGATCTGAACTTTTTATCACATTGCAACCT1087910.6299967420451409No Hit
TATCGAACTATTGATGAACTTTAACATCTT815560.47228184587174965No Hit
TATCACAGCTGGCTTGAGTGAGC690370.3997856907333363No Hit
TGGACGGAGAACTGATAAGGGT498030.2884037075132516No Hit
TGATCTGAACTTTTTATCACATTTAAGCT449020.2600225543593764No Hit
TGATCTGAACTTTTTATCACATTGCAACC410730.23784923556194973No Hit
TTCGACTAGGAGATTTGC409180.23695164757197817No Hit
TTTTACATCTTTGTGACGTCTGCCAGAGC383750.22222541364618656No Hit
TCGACTAGGAGATTTGT371660.21522422732440835No Hit
TGTGATCTTCTGGGAAATAGATAATTCCGT337020.19516458347110827No Hit
TGTATCGGTCTTTTGGTGCTTACACCTGG325490.18848768700377136No Hit
TGATCTGAACTTTTTATCACATTGCAACCA289010.16736251934302118No Hit
TGTATCGGTCTTTTGGTGCTTACACCTGGT288310.1669571570249695No Hit
TAAGATCTCTAGATTATCCATTTTGTCTG263520.1526015400756823No Hit
CAACTCTATGCGGTGGATCACTCGGCTCGTG240280.13914351111636666No Hit
TGAATCAAGATTTGTGTTCTTTTGTGAATC227430.13170221713498947No Hit
TTTGTCTAGGATGTTGTGAGGTTTCATTTT221700.1283840370172236No Hit
TTGACTTAAACAATACTCTGGAGTGTATCC217330.1258534179745296No Hit
TAAGGGACTGGCAAGTATCTGCTCTATGCC214520.12422617781206499No Hit
TTAGATCTCGAGTAGTATTTGTTTACAGG211780.12263947388140557No Hit
TGACGTTGAATTGGAAATAATGAAGATCC192110.11124879274415349No Hit
TGAATGTCTTTGGTTACTTTTGTAAACTT190840.1105133496814026No Hit
TATCGAACTATTGATGAACTTTAACATCT185000.10713146977080004No Hit
CAGGACAGGTTTACAATATTTTATTAATGT181870.10531892112008326No Hit
TTTGTGCTCTGTTTTGACCTCAATGAAGGC180710.10464717785016907No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)