FastQCFastQC Report
Thu 5 Dec 2024
35-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename35-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14222001
Total Bases405.7 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGACTAGGAGATTTGT3325602.3383488722859744No Hit
TGATCTGAACTTTTTATCACATTGCAACCC1602121.1265081474822003No Hit
TATCACAGCTGGCTTGAGTGAGC943610.6634861015689706No Hit
TGATCTGAACTTTTTATCACATTGCAACCT644630.4532625191068402No Hit
TATCGAACTATTGATGAACTTTAACATCTT524250.36861901500358496No Hit
TGGACGGAGAACTGATAAGGGT512950.3606735789148095No Hit
TGATCTGAACTTTTTATTACATTGCAACCC465290.3271621201545409No Hit
TTCGACTAGGAGATTTGC385040.27073546120549424No Hit
TGGTCTAGTTTTCTTTGAAGTAATTGAAGT346410.24357331995687528No Hit
TCTATGTCTCTTGTGTTCTTTATGAAGATT336400.23653492922690697No Hit
TATCGAACTATTGATGAACCTTAACATCTT316520.22255658679815873No Hit
TAAGGTCTGAAGCTCCTCATTACAAGAGCAC299050.21027280197772452No Hit
TGCAGGACATTCAATTCTCTGGCAAGACTG297860.2094360702126234No Hit
TCGACTAGGAGATTTGT250420.17607930135850786No Hit
TAAGATCTCTAGATTATCCATTTTGTCTG247730.17418786568781708No Hit
TGAACTAGATCTGTAGACTTCCCAACAGGT227750.16013920966536282No Hit
TCCAGCTTCGTATCTGTTTATAAATTCAGC227710.16011108422788045No Hit
TTTGTCTAGGATGTTGTGAGGTTTCATTTT209070.14700463036108632No Hit
TGAATACTCGGTCTAAAGCTTCAACAATTT205820.14471943856564207No Hit
TGTGATCTTCTGGGAAATAGATAATTCCGT205730.14465615633130668No Hit
TATCACAGCTGGCTTGAGTGAGT201000.14133032334901396No Hit
TGATCTGAACTTTTTATCACATTTAAGCC200850.14122485295845502No Hit
TGATCTGAACTTTTTATCACATTGCAACCA196080.1378708945386799No Hit
TGATCTGAACTTTTTATTACATTGCAACCT181840.12785823879494876No Hit
TGATCTAAATTAATAGCATGTGACTGTTGT175300.12325972976657786No Hit
TAAGACTGTATCTGAAGCTATGTTGGGCAT171520.12060187592449192No Hit
TGGATCCAATAGAAATTATCTGTTCATT167910.11806355519170615No Hit
AATTGCACTTGTCCCGGCCTGC167460.11774714402002925No Hit
TATCACAGCCTGCTTTGATGAGC153330.10781183322937468No Hit
ATTGGTCGAACTGGTTATCAGCTTTT152530.10724932447972688No Hit
TGAACGAACTCTGAAATACACACAATCATG150480.10580789580875434No Hit
TGTGAATAGATCTGCGGTCTTTGAACCAC142900.10047812540584126No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)