FastQCFastQC Report
Thu 5 Dec 2024
341-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename341-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15703739
Total Bases446.9 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGACTAGGAGATTTGT4623842.9444197970941826No Hit
TATCACAGCTGGCTTGAGTGAGC870030.5540272924811092No Hit
TATCGAACTATTGATGAACTTTAACATCTT669460.4263061172883732No Hit
TGTGATCTTCTGGGAAATAGATAATTCCGT544980.3470383709255484No Hit
TTCGACTAGGAGATTTGC508600.3238719135614773No Hit
TGGACGGAGAACTGATAAGGGT492460.3135941064736239No Hit
TGATCAACGTTCGTACCATAAAAGAACAAGT489030.31140991326969963No Hit
TCGACTAGGAGATTTGT481770.30678681045323025No Hit
TCTATGTCTCTTGTGTTCTTTATGAAGATT456890.29094344983701015No Hit
TCTAGTCTTTCCAGTGTAGAAGCTGAGGTT328670.20929410505357993No Hit
TGTATCGGTCTTTTGGTGCTTACACCTGGT327630.20863184239116556No Hit
TGTATCGGTCTTTTGGTGCTTACACCTGG321180.20452454030215353No Hit
TACGTGAAATCGCTCAGGACTTCAAGACTG259760.16541283575841398No Hit
TAAGTCTGTGCTTGTGAATCTGGTCTGTCC258010.16429845147069752No Hit
TAAGTCTGTGCTTTTGGATCTGGTCTGTAC235740.15011711542072878No Hit
TCATCTTCAGTATATCTAACTTTGAACAAGT215280.13708837111976963No Hit
TATCACAGCTGGCTTGAGTGAGT204670.1303320183811002No Hit
TACAGTTGACGGTCTTCTACTCGAGAGAGG203550.12961881243696166No Hit
CAACTCTATGCGGTGGATCACTCGGCTCGTG199420.12698886551795086No Hit
TATTAACTACTCTATTGTTCGTCACGGTGT198810.12660042299480398No Hit
TATGTCCGGGCTATAGAGAGAACCTCTTCAT193720.123359156695103No Hit
ATTGGTCGAACTGGTTATCAGCTTTT184360.11739879273337386No Hit
TCCGATCGTTAAGACAATGATGTCCAAAGC170670.1086811236483235No Hit
TAAGATCTCTAGATTATCCATTTTGTCTG166610.10609575210082133No Hit
TGAGGATCTGTGCATTGGCGTGATTTAAAC166570.10607028045995924No Hit
TTTGAACATCTTGAGAAGTAGCTTATCTTT166540.10605117672931269No Hit
TAAGTCTGTGTTTTTGGATCTGGTCTATCC166120.10578372450026072No Hit
TCTTTCCTCATCTGACATTTCATTGACATC165590.10544622525883805No Hit
TGATCTGAACTTTTTATCACATTGCAACCC161290.10270802386616333No Hit
TAGAACATCTGGATGGATTCTTATAAAATTC159580.10161911121930897No Hit
TCAGACTGTCTGTCAGAAAATAAAGCAGC158440.1008931694547394No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)