FastQCFastQC Report
Thu 5 Dec 2024
34-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename34-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18959533
Total Bases543.3 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGACTAGGAGATTTGT3626591.9128055527528027No Hit
TGATCTGAACTTTTTATCACATTGCAACCC1678790.8854595732922325No Hit
TATCACAGCTGGCTTGAGTGAGC1071530.5651668740996942No Hit
TGATCTGAACTTTTTATCACATTGCAACCT613030.32333602309719334No Hit
TATCGAACTATTGATGAACTTTAACATCTT611280.3224130045819167No Hit
TGATCTGAACTTTTTATCACATTTAAGCC592300.31240220948480113No Hit
TTCGACTAGGAGATTTGC434060.22894023813772205No Hit
TGGACGGAGAACTGATAAGGGT427220.22533255434086905No Hit
TCTATGTCTCTTGTGTTCTTTATGAAGATT327380.17267302944645313No Hit
CAACTGTTGATTTCTGAGGGCTAACCAAAG308050.16247763064628226No Hit
TGATCTGAACTTTTTATTACATTGCAACCC292940.15450802506580727No Hit
TTTGTCTCGGTTCGCCATCTGGATGGAATG261340.1378409478756676No Hit
TCCGATCGTTAAGACAATGATGTCCAAAGC260490.13739262459681892No Hit
TGTGATCTTCTGGGAAATAGATAATTCCGT256850.13547274608504334No Hit
TGTATCGGTCTTTTGGTGCTTACACCTGG246410.12996628134247823No Hit
TCCTCTTTCACTGATTGCAACTCTGTAGTC222370.11728664413833399No Hit
TCCAGCTTCGTATCTGTTTGTAAATTCAGC211190.1113898744235947No Hit
TATCACAGCTGGCTTGAGTGAGT207170.1092695690342162No Hit
TAAGGTCTGAAGCTCCTCATTACAAGAGCAC203510.10733914173940888No Hit
TATCACAGCCTGCTTTGATGAGC199410.10517664121790342No Hit
TCATCTTCAGTATATCTAACTTTGAACAAGT198750.1048285313778562No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)