FastQCFastQC Report
Thu 5 Dec 2024
33-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename33-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16654275
Total Bases477 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGACTAGGAGATTTGT2798051.6800791388397276No Hit
TGATCTGAACTTTTTATCACATTGCAACCC2518021.511936124508572No Hit
TATTTGGTCTGCAAAGTTGTCTTTATAGC1726691.0367848495356298No Hit
TATCGAACTATTGATGAACTTTAACATCTT1101690.6615058295842959No Hit
TGATCTGAACTTTTTATCACATTGCAACCT888430.5334546235125817No Hit
TATCACAGCTGGCTTGAGTGAGC882230.5297318556346644No Hit
TGATCTGAACTTTTTATCACATTTAAGCC629120.377752859250853No Hit
TCTATGTCTCTTGTGTTCTTTATGAAGATT515960.3098063410145443No Hit
TGGACGGAGAACTGATAAGGGT441860.2653132604091142No Hit
TGTAGTTCTTCCAGATGTCTCAGATATTG416120.24985776925143843No Hit
TGATCAACGTTCGTACCATAAAAGAACAAGT374830.2250653360773735No Hit
TTCGACTAGGAGATTTGC326060.19578156359253107No Hit
TATATGTGAACCGTCGGGTATTTTGGACC323740.1943885278704717No Hit
TATTTGGTCTGCAAAGTTGTCTTTATAGT304930.18309413048601636No Hit
TGATCTGAACTTTTTATCACATTGCAACCA275490.16541698753022874No Hit
TAAGGTCTGTGTTTGTTTCATAAAAACATG262130.15739502319974902No Hit
TGATCTGAACTTTTTATCACATTGCAACC258810.1554015410457675No Hit
TCACTGGTCTGGTGGCGTGTAATGGCCTTC229650.1378925230909181No Hit
TGGATCCAATAGAAATTATCTGTTCATT226740.1361452239740247No Hit
TCTAGTCTTTCCAGTGTAGAAGCTGAGGTT222070.1333411391369483No Hit
TAAGATCTCTAGATTATCCATTTTGTCTG214500.12879575964729778No Hit
TACGAATATCCGGATCTGAAAACTGAATTC213840.12839946500222915No Hit
TGATCTGAACTTTTTATTACATTGCAACCC200690.12050359442245309No Hit
TGGTCTAGTTTTCTTTGAAGTAATTGAAGT199530.11980707656142342No Hit
TCGGGAATATCACTCTCTACTGAGTCAAGT199190.1196029247745699No Hit
TCGACTAGGAGATTTGTT192490.1155799336806916No Hit
CAACTCTATGCGGTGGATCACTCGGCTCGTG185990.11167703187319772No Hit
TACGTGAAATCGCTCAGGACTTCAAGACTG185100.11114263454878703No Hit
TTTAGATCTCTGGTATATTTATTTTGTCTC183400.11012187561451939No Hit
TGATCTGAACTTTTTATCACATTTAAGCT181830.10917917471640164No Hit
AATTGCACTTGTCCCGGCCTGC175150.10516819255116179No Hit
TCGACTAGGAGATTTGT170180.10218397378450879No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)