FastQCFastQC Report
Thu 5 Dec 2024
310-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename310-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15957669
Total Bases453.3 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGACTAGGAGATTTGT3879092.4308625526698164No Hit
TGATCTGAACTTTTTATCACATTGCAACCC2328511.459179282387672No Hit
TATCACAGCTGGCTTGAGTGAGC1115380.698961734323478No Hit
TGATCTGAACTTTTTATCACATTGCAACCT915080.5734421487248546No Hit
TGATCTGAACTTTTTATCACATTTAAGCC702680.440340002039145No Hit
TGGACGGAGAACTGATAAGGGT658150.412434923922786No Hit
TATCGAACTATTGATGAACTTTAACATCTT530420.3323919051084466No Hit
TCTATGTCTCTTGTGTTCTTTATGAAGATT516060.32339309707451636No Hit
TTCGACTAGGAGATTTGC416840.2612160961604104No Hit
TCGACTAGGAGATTTGT411610.2579386751285542No Hit
TGATCTGAACTTTTTATTACATTGCAACCC324980.20365129769266427No Hit
TGTCTGTCGGCATTTGACGGCATCTGCCAC296610.18587301190418223No Hit
TGATCTGAACTTTTTATCACATTGCAACC280230.17560835482926734No Hit
TATCACAGCTGGCTTGAGTGAGT263390.16505543510145498No Hit
TTTTCCATGTTCTCTCTGATATTTGAAGTT246700.15459651406480485No Hit
TGTGTCTTTATCGCTTTGTACAATAGTAGT245300.15371919294729072No Hit
TGATCTGAACTTTTTATCACATTTAAGCT233410.14626823002783174No Hit
TGGCTATATCATCAGGATCTGTGAATAT232860.14592356816023694No Hit
TCCGATCGTTAAGACAATGATGTCCAAAGC226250.14178135916968826No Hit
TGATCTGAACTTTTTATCACATTGCAACCA223730.14020218115816288No Hit
AATTGCACTTGTCCCGGCCTGC211280.13240028979169827No Hit
TGTACGAGCTGGAGCACTTGAACGCTTTC207590.13008792198910757No Hit
TATCACAGCCTGCTTTGATGAGC183970.11528626142076265No Hit
TCCGTCTGTCAGATTGTGTTGTAGTGTC178070.11158897956838182No Hit
TGTATCGGTCTTTTGGTGCTTACACCTGG177810.11142604850370064No Hit
CAACTCTATGCGGTGGATCACTCGGCTCGTG176910.1108620563567273No Hit
TGTGTCTAAATCATGTAACTTTGATCATTT164390.10301629893438696No Hit
TATCTACTCAGGACTGGACTACCTTTAGT163030.10216404413451614No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)