FastQCFastQC Report
Thu 5 Dec 2024
30-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename30-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16076861
Total Bases459.5 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGACTAGGAGATTTGT2823801.7564374040429906No Hit
TGATCTGAACTTTTTATCACATTGCAACCC2716101.6894467147535828No Hit
TATCACAGCTGGCTTGAGTGAGC1172600.7293712373329595No Hit
TGATCTGAACTTTTTATCACATTGCAACCT1029090.6401062993578162No Hit
TGATCTGAACTTTTTATCACATTTAAGCC914490.568823727467694No Hit
TGGACGGAGAACTGATAAGGGT730930.45464721004927516No Hit
TGTCTGTCGGCATTTGACGGCATCTGCCAC383360.23845450924779407No Hit
TTCGACTAGGAGATTTGC334560.208100325057236No Hit
TGATCTGAACTTTTTATCACATTGCAACCA316630.19694765041509038No Hit
TGATCTGAACTTTTTATCACATTGCAACC313300.19487635055126742No Hit
TACGTTGTGTTTCTTTCGGTTGTAAAAGC293570.1826040543611094No Hit
TGCAGGACATTCAATTCTCTGGCAAGACTG289120.17983610108963435No Hit
TGATCTGAACTTTTTATCACATTTAAGCT282380.17564374040429906No Hit
CAACTCTATGCGGTGGATCACTCGGCTCGTG279100.17360354113903206No Hit
TATGTCCGGGCTATAGAGAGAACCTCTTC277830.1728135859357122No Hit
TATGTCCGGGCTATAGAGAGAACCTCTTCAT269420.16758246525860987No Hit
TTGTAGAACTGTCTTACTATCTTGTAGAGC247400.15388576165459167No Hit
TGGATCCAATAGAAATTATCTGTTCATT246920.15358719590845502No Hit
TATCACAGCTGGCTTGAGTGAGT230930.1436412244902783No Hit
TGAATACTCGGTCTAAAGCTTCAACAATTT229660.14285126928695846No Hit
TATCGAACTATTGATGAACTTTAACATCTT229150.14253404318168825No Hit
TAAGATCTCTAGATTATCCATTTTGTCTG217910.13554262862632202No Hit
TATCACAGCCTGCTTTGATGAGC202420.12590766319370428No Hit
AATTGCACTTGTCCCGGCCTGC197210.12266698082417954No Hit
TTGACTTAAACAATACTCTGGAGTGTATCC192060.11946361917292189No Hit
TGAAACAGACGTTGTAGGAGTTGAAACTTC191270.11897222971573866No Hit
TCTATGTCTCTTGTGTTCTTTATGAAGATT179860.11187507312528235No Hit
CGAGAACTCTTTGGAGAAAATCCTCCTCAG179600.11171335001279167No Hit
TCCGAGAAACTGTTGTACACATTGTATAC176590.10984109397972651No Hit
TCCGATCGTTAAGACAATGATGTCCAAAGC176280.10964827026867993No Hit
TGAAGTCTTCTACACTGTGGTCTGTAGTGT174680.10865305111489114No Hit
TGAATCAAGATTTGTGTTCTTTTGTGAATC171340.10657553113135705No Hit
TGTAGAAATCTCTTGATCATCTTGGTTTTG163060.10142527201050006No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)