FastQCFastQC Report
Thu 5 Dec 2024
260-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename260-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15809273
Total Bases448.4 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGACTAGGAGATTTGT3489322.2071350150003735No Hit
TGATCTGAACTTTTTATCACATTGCAACCC1589341.005321370565237No Hit
TGATCTGAACTTTTTATCACATTTAAGCC1100380.6960345361864521No Hit
TATCACAGCTGGCTTGAGTGAGC895180.5662372963007217No Hit
TGATCTGAACTTTTTATCACATTGCAACCT608880.38514104981298No Hit
TTTGTCTCGGTTCGCCATCTGGATGGAATG556160.3517935328208957No Hit
TGGACGGAGAACTGATAAGGGT512520.3241894804397394No Hit
TTCGACTAGGAGATTTGC426610.2698479556903091No Hit
TGTATCGGTCTTTTGGTGCTTACACCTGG409160.2588101299787789No Hit
TGATCTGAACTTTTTATCACATTGCAACC354010.2239255404091004No Hit
TGATCTGAACTTTTTATCACATTTAAGCT339190.21455129530624212No Hit
TCGACTAGGAGATTTGT314080.19866821200443563No Hit
TCACTGGTCTGGTGGCGTGTAATGGCCTTC306610.19394313704368316No Hit
TAAGATCTCTAGATTATCCATTTTGTCTG261230.1652384647921508No Hit
TGGTCTAGTTTTCTTTGAAGTAATTGAAGT259520.16415682112643637No Hit
TCCGAGAAACTGTCGTACACATTGTATAC251090.1588245076165109No Hit
CAACTCTATGCGGTGGATCACTCGGCTCGTG215030.1360151096131998No Hit
TAAGGTCTGTGTTTGTTTCATAAAAACATG206460.1305942404815199No Hit
TGGCTATATCATCAGGATCTGTGAATAT198050.12527457777470224No Hit
TTTTCCATGTTCTCTCTGATATTTGAAGTT197630.12500891090943905No Hit
TGATCTGAACTTTTTATCACATTGCAACCA192250.12160584487344864No Hit
AATTGCACTTGTCCCGGCCTGC188400.11917056527520273No Hit
TGTGATCTTCTGGGAAATAGATAATTCCGT180270.11402801381189381No Hit
TATACGTTTTGGGACATTTGCAACATTTCC172540.10913847841074033No Hit
TATCACAGCTGGCTTGAGTGAGT172410.10905624819053981No Hit
TTTGTCTCGGTTCGCCATCTGGATGGCATG170880.10808846175279534No Hit
TGATCTGAACTTTTTATTACATTGCAACCC168710.10671584961560218No Hit
TATCACAGCCTGCTTTGATGAGC159760.10105461522487466No Hit
TGATTTAAAGTCATCTGAACGGTCCCTTT159400.10082690076893479No Hit
TGTATCGGTCTTTTGGTGCTTACACCTGGT158960.10054858310056382No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)