FastQCFastQC Report
Thu 5 Dec 2024
26-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename26-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14609475
Total Bases417 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGACTAGGAGATTTGT3112772.130651512117992No Hit
TATCACAGCTGGCTTGAGTGAGC955050.6537195895129702No Hit
TGGACGGAGAACTGATAAGGGT551700.37763163974064773No Hit
TTCGACTAGGAGATTTGC378550.25911266489726703No Hit
CAACTCTATGCGGTGGATCACTCGGCTCGTG333890.22854346237630033No Hit
TATCGAACTATTGATGAACTTTAACATCTT305130.20885760781958282No Hit
TTTGTCTCTGTTCGCCATCTGGATGGCATG303960.2080567576863645No Hit
TACGACTGTACTTGTCATCTCTCGACAATC292730.20036996538205515No Hit
TAAGATCTCTAGATTATCCATTTTGTCTG270790.18535231416597792No Hit
TATCTACTCAGGACTGGACTACCTTTAGT261240.17881546051449487No Hit
TGGCTATATCATCAGGATCTGTGAATAT257990.1765908768111106No Hit
TGGTCTAGTTTTCTTTGAAGTAATTGAAGT257530.17627601265617004No Hit
TTGACGTAATTGACTTTGACCTATATTTTT226940.1553375463526239No Hit
TGAATACTCGGTCTAAAGCTTCAACAATTT226610.15511166554581873No Hit
TCGACTAGGAGATTTGT223530.15300344468230379No Hit
TCGACTTCTAGATGAGCTCATTTATCCAGG215420.1474522527332433No Hit
TAAAAACCAGACTGCTCTCCTTCTTCACAGC208000.14237335701659368No Hit
TGAAGACGTTGAAGATCTTGATGCCATTG198010.1355353289560371No Hit
TCAGATCTGTCATATTCCAACTTATGT196340.13439223517614424No Hit
CAAGTCTTGTCAGGACGTGTATGCACCGTT195740.13398154280013483No Hit
TCAATGATCTCCTACATCTCTGAAAAAGG195350.13371459275572872No Hit
TCATAGTGTCTAGGATACTGTAGTCTCTT191150.1308397461236629No Hit
TGTAGAAATCTCTTGATCATCTTGGTTTTG184780.12647956206502972No Hit
TGATCTGAACTTTTTATCACATTGCAACCC183850.12584298888221515No Hit
TAAGGTCTGAAGCTCCTCATTACAAGAGCAC182210.12472042972112277No Hit
TATGTCCGGGCTATAGAGAGAACCTCTTC182070.12462460150005392No Hit
TATTTGGTCTGCAAAGTTGTCTTTATAGC176870.12106526757463906No Hit
AATTGCACTTGTCCCGGCCTGC175620.1202096584579528No Hit
TATGTGGACTATTGTGCCTTTGGTGCTTT171670.11750593364922422No Hit
TCAATAGCCTGCTCTAAATTGTAGAAATTT167530.11467215625475932No Hit
TGTGCTTTTGAACCTGTTCTAAAAATGTTC163950.11222169174456988No Hit
TATCACAGCTGGCTTGAGTGAGT160350.10975753748851344No Hit
TGTATTCTCAATGTAGTTTATCTAGGCGT155920.10672525877897733No Hit
TGGTGAAGAAGGCTCTGATGAAACAGACGT154120.10549318165094913No Hit
TATTACGATTGCTGGTAACGTAGAGCCTGC150660.10312485561596156No Hit
TATCACAGCCTGCTTTGATGAGC150040.10270047349408516No Hit
ATTGGTCGAACTGGTTATCAGCTTTT147710.101105618100582No Hit
TGATGTTGTGAAGACCCTACTGTCAGTGT147090.1006812359787056No Hit
TGTTATCTCACGATCATCTGAAGCTTCTTC146200.10007204228762498No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)