FastQCFastQC Report
Thu 5 Dec 2024
24-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename24-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15464670
Total Bases437.3 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGACTAGGAGATTTGT3912032.529656306924105No Hit
TATTTGGTCTGCAAAGTTGTCTTTATAGC1848491.1952987034317577No Hit
TATCACAGCTGGCTTGAGTGAGC988460.6391730311736364No Hit
TGTATCGGTCTTTTGGTGCTTACACCTGG532930.34461129788091177No Hit
TGAATACTCGGTCTAAAGCTTCAACAATTT500460.3236150528915263No Hit
TCGACTAGGAGATTTGTT437130.28266364558700574No Hit
TTCGACTAGGAGATTTGC430630.2784605167779202No Hit
TATCTACTCAGGACTGGACTACCTTTAGT400070.25869934502320446No Hit
TATTTGGTCTGCAAAGTTGTCTTTATAGT399310.25820790227014223No Hit
TGTGATCTTCTGGGAAATAGATAATTCCGT399020.25802037806173683No Hit
TCTGATCTCTTGGCTGTTTTTAGAGCTTG367970.23794235505833616No Hit
TATGTCCGGGCTATAGAGAGAACCTCTTC343860.22235198035263604No Hit
TTTGTCTCTGTTCGCCATCTGGATGGCATG343440.22208039356804896No Hit
TGGACGGAGAACTGATAAGGGT322600.2086045159709195No Hit
TCCGAGAAACTGTTGTACACATTGTATAC283430.183275815132169No Hit
TCGACTAGGAGATTTGT279400.18066987527053602No Hit
TATCGAACTATTGATGAACTTTAACATCTT271390.17549032730733988No Hit
TTTTCCATGTTCTCTCTGATATTTGAAGTT225980.1461266228118673No Hit
TTCGACTAGGAGATTTGTT223810.14472342442483416No Hit
TATCACAGCTGGCTTGAGTGAGT212240.137241855144662No Hit
TACGTGAAATCGCTCAGGACTTCAAGACTG204520.13224983138987123No Hit
TTCGACTAGGAGATTTGG197140.12747766360355572No Hit
TATCACAGCCTGCTTTGATGAGC187980.12155448515875217No Hit
TGAACGAACTCTGAAATACACACAATCATG182800.11820491481551174No Hit
TATACGTTTTGGGACATTTGCAACATTTCC182670.11812085223933003No Hit
TCACTGGTCTGGTGGCGTGTAATGGCCTTC173130.11195195241799534No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)