FastQCFastQC Report
Thu 5 Dec 2024
211-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename211-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14331615
Total Bases406.4 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGACTAGGAGATTTGT3786092.6417748453332024No Hit
TATCACAGCTGGCTTGAGTGAGC1131930.7898132904072569No Hit
TGATCTGAACTTTTTATCACATTGCAACCC1063500.7420657057840306No Hit
TGATCTGAACTTTTTATCACATTTAAGCC693400.4838254446550511No Hit
TGGACGGAGAACTGATAAGGGT508780.35500535005998973No Hit
TTCGACTAGGAGATTTGC421500.2941050258467033No Hit
TGATCTGAACTTTTTATCACATTGCAACCT407880.2846015609545749No Hit
TATCGAACTATTGATGAACTTTAACATCTT378530.26412236164591363No Hit
TGTGTCTAAATCATGTAACTTTGATCATTT291530.20341740969178979No Hit
TGAACTAGATCTGTAGACTTTCCAACAGGT257850.17991691794679104No Hit
TATCACAGCTGGCTTGAGTGAGT239160.166875819647681No Hit
TAAGGTCTGAAGCTCCTCATTACAAGAGCAC237890.1659896669007645No Hit
TAAGATCTCTAGATTATCCATTTTGTCTG231000.16118211380922529No Hit
TGATCTGAACTTTTTATCACATTTAAGCT219170.1529276358596013No Hit
TCGGGAATATCACTCTCTACTGAGTCAAGT196930.13740949641753564No Hit
TCGACTAGGAGATTTGT193990.13535808769632732No Hit
TTAAGTATATTGTTGTGTAGAATCAATT189200.13201582654850832No Hit
TTTGTCTCGGTTCGCCATCTGGATGGCATG188870.1317855663859237No Hit
TATCACAGCCTGCTTTGATGAGC188830.13175765606318618No Hit
TCCGATCGTTAAGACAATGATGTCCAAAGC187020.13049471395931303No Hit
TGTGATCTTCTGGGAAATAGATAATTCCGT171410.11960271051099265No Hit
TGGTAAGATTTGGTTGTGACTGCATAACC170220.1187723784095512No Hit
AATTGCACTTGTCCCGGCCTGC168920.11786529292058152No Hit
TCTAGTCTTTCCAGTGTAGAAGCTGAGGTT161340.11257628676181992No Hit
CTTTCGACTAGGAGATTTGT160460.11196225966159432No Hit
TGATTTAATTTGTGACTGTTTTGCTGATT150120.10474744123394328No Hit
TGTATCGGTCTTTTGGTGCTTACACCTGG144720.10097954766437697No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)