FastQCFastQC Report
Thu 5 Dec 2024
199-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename199-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13501036
Total Bases384 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGACTAGGAGATTTGT3591532.6601884477606017No Hit
TGATCTGAACTTTTTATCACATTGCAACCC1094240.8104859508559196No Hit
TATCACAGCTGGCTTGAGTGAGC1009900.7480166707206766No Hit
TGGACGGAGAACTGATAAGGGT595810.4413068745242958No Hit
TGATCTGAACTTTTTATCACATTTAAGCC529030.3918440036749772No Hit
TGATCTGAACTTTTTATCACATTGCAACCT420710.3116131236151063No Hit
TTCGACTAGGAGATTTGC418980.3103317404679167No Hit
TAAGGTCTGAAGCTCCTCATTACAAGAGCAC245870.18211195051994528No Hit
TATGTCCGGGCTATAGAGAGAACCTCTTC243060.18003062876063733No Hit
TATGTCCGGGCTATAGAGAGAACCTCTTCAT230520.17074245265326304No Hit
TCAGATCTGAATAACTCAATATGAACAAGTC230260.17054987483923456No Hit
TATCACAGCTGGCTTGAGTGAGT213210.15792121434236603No Hit
TATGTCTGTTCATGCATCCCTCTGTATGTC200700.14865525875199503No Hit
TGATTTAATTTGTGACTGTTTTGCTGATT200620.14859600403998627No Hit
TCTATGTCTCTTGTGTTCTTTATGAAGATT192520.14259646444909857No Hit
TCGACTAGGAGATTTGT188060.1392930142546098No Hit
TTTTGTCTCTTGATTGATCTGCTAAAAGC179320.13281943696765197No Hit
TCCGAGAAACTGTTGTACACATTGTATAC178980.1325676044416147No Hit
TATCACAGCCTGCTTTGATGAGC167910.12436823366740152No Hit
TACGTGAAATCGCTCAGGACTTCAAGACTG164820.12207952041606289No Hit
TGATCTGAACTTTTTATCACATTTAAGCT162010.11999819865675494No Hit
TATCTACTCAGGACTGGACTACCTTTAGT158210.11718359983633848No Hit
AATTGCACTTGTCCCGGCCTGC136760.10129593017898775No Hit

[OK]Adapter Content

Adapter graph