FastQCFastQC Report
Thu 5 Dec 2024
196-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename196-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16412799
Total Bases457.4 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGACTAGGAGATTTGT5782473.5231467831903625No Hit
TGATCTGAACTTTTTATCACATTGCAACCC1878651.1446249966261086No Hit
TATCACAGCTGGCTTGAGTGAGC783470.4773530706127577No Hit
TGATCTGAACTTTTTATCACATTGCAACCT750200.45708230509616304No Hit
TTCGACTAGGAGATTTGC662860.40386773761136047No Hit
TATTTGGTCTGCAAAGTTGTCTTTATAGC621240.3785094790961615No Hit
TGGACGGAGAACTGATAAGGGT564380.34386578425776126No Hit
TGATCTGAACTTTTTATCACATTTAAGCC498630.3038055848974937No Hit
TACGTTGTGTTTCTTTCGGTTGTAAAAGC450980.27477336437252414No Hit
CTTTCGACTAGGAGATTTGT356380.21713541974162967No Hit
TCGACTAGGAGATTTGT317320.1933369195589369No Hit
TAAGATCTCTAGATTATCCATTTTGTCTG293070.17856186504203214No Hit
TGAAGACGTTGAAGATCTTGATGCGATTG252470.15382507273744106No Hit
TGATCTGAACTTTTTATCACATTGCAACC245040.14929811788958117No Hit
GAGAAAAGCGGGGCATGGCTAGACTTC232380.14158462551085893No Hit
TGATCTGAACTTTTTATCACATTGCAACCA228280.13908657505645441No Hit
TGCCGAATTGTAGTCTATAGAAGC197590.12038775348433865No Hit
TATGTCCGGGCTATAGAGAGAACCTCTTC184790.11258896182180747No Hit
TCACTGGTCTGGTGGCGTGTAATGGCCTTC181360.11049912936848857No Hit
TGTATCGGTCTTTTGGTGCTTACACCTGG179900.10960957969448112No Hit
TATCACAGCCTGCTTTGATGAGC177320.10803763575000219No Hit
TATCACAGCTGGCTTGAGTGAGT172390.1050338823987304No Hit
TACGTGAAATCGCTCAGGACTTCAAGACTG168070.10240179021262615No Hit
TACTGTCTCTCAATCTGAATTGAATTTTC167940.10232258373480355No Hit
AATTGCACTTGTCCCGGCCTGC164940.10049474193889782No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)