FastQCFastQC Report
Thu 5 Dec 2024
88_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename88_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22568598
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAATCTCCTAGTCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA5917892.6221788345027015Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA4551552.0167624058880396Illumina RNA PCR Primer (100% over 45bp)
ACAAATCTCCTAGTCGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAG2818511.2488635758410869Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGATCGTC1683580.7459834235161616No Hit
AAGATGTTAAAGTTCATCAATAGTTCGATAGATCGTCGGACTGTAGAACT1630940.722658979525445No Hit
GGGTTGCAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACT956960.4240227948585907No Hit
GCTCACTCAAGCCAGCTGTGATAGATCGTCGGACTGTAGAACTCTGAACG899540.39858036374257716Illumina Small RNA Adapter 1 (100% over 26bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT885160.3922086786250524Illumina RNA PCR Primer (100% over 50bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGGATCGT702070.31108268222952973No Hit
GCAAATCTCCTAGTCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA681900.3021454855104424Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCTCTGGCAGACGTCACAAAGATGTAAAAGATCGTCGGACTGTAGAACTC551430.2443350712348193Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACCCTTATCAGTTCTCCGTCCAGATCGTCGGACTGTAGAACTCTGAACGT523740.23206581108848676Illumina DpnII expression Sequencing Primer (96% over 28bp)
AATCTTCATAAAGAACACAAGAGACATAGAGATCGTCGGACTGTAGAACT490830.21748360265888025No Hit
GTGCTCTTGTAATGAGGAGCTTCAGACCTTAGATCGTCGGACTGTAGAAC476370.2110764700580869No Hit
GAAGAGGTTCTCTCTATAGCCCGGACATAGATCGTCGGACTGTAGAACTC410370.18183229636151965Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGTGATCG395160.17509284360508348No Hit
AGGTTGCAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACT382350.1694168153467043No Hit
GCAAATCTCCTAGTCGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAG319810.14170574530150257Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACATACAGAGGGATGCATGAACAGACATAGATCGTCGGACTGTAGAACT291140.1290022534851301No Hit
ATGAAGAGGTTCTCTCTATAGCCCGGACATAGATCGTCGGACTGTAGAAC270300.11976818409366857No Hit
ATGCCCAACATAGCTTCAGATACAGTCTTAGATCGTCGGACTGTAGAACT250300.11090631327652697No Hit
GTTCAGTGAGATTTGTCAACATGAAAATTCTGACCCGAAAGATGGACATC241840.10715774192087608No Hit
CAGTCTTGAAGTCCTGAGCGATTTCACGTAGATCGTCGGACTGTAGAACT241560.1070336757294361No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)