FastQCFastQC Report
Thu 5 Dec 2024
88_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename88_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22568598
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC66

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACTAGGAGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA5694582.523231615893907Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTGGGGGG3100641.3738735565230946TruSeq Adapter, Index 8 (97% over 45bp)
TTCGACTAGGAGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC2695441.194332053767806Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATCGG1642180.7276393509246786No Hit
TATCGAACTATTGATGAACTTTAACATCTTAGATCGGAAGAGCACACGTC1589440.7042705975798763No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTAGGGGG985610.43671742480414605TruSeq Adapter, Index 8 (97% over 45bp)
TGATCTGAACTTTTTATCACATTGCAACCCAGATCGGAAGAGCACACGTC975340.4321668541395438No Hit
TATCACAGCTGGCTTGAGTGAGCAGATCGGAAGAGCACACGTCTGAACTC928080.4112262533986382Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATCG688660.3051407978466363No Hit
TCGACTAGGAGATTTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA686280.30408623521939643Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTTTACATCTTTGTGACGTCTGCCAGAGCAGATCGGAAGAGCACACGTCT547670.24266903952119664Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAACTCTATGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAGCGCAGC521280.23097580097797832No Hit
TGGACGGAGAACTGATAAGGGTAGATCGGAAGAGCACACGTCTGAACTCC504730.2236426028767937Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAAGGTCTGAAGCTCCTCATTACAAGAGCACAGATCGGAAGAGCACACGT489370.21683668608922896No Hit
TCTATGTCTCTTGTGTTCTTTATGAAGATTAGATCGGAAGAGCACACGTC472110.20918889157403572No Hit
TATGTCCGGGCTATAGAGAGAACCTCTTCAGATCGGAAGAGCACACGTCT416470.18453516696074784Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATC387820.17184053701519253No Hit
TGATCTGAACTTTTTATCACATTGCAACCTAGATCGGAAGAGCACACGTC360090.15955355312722572No Hit
TTCGACTAGGAGATTTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC320950.14221087193807963Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATGTCTGTTCATGCATCCCTCTGTATGTCAGATCGGAAGAGCACACGTC293930.13023848446412134No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTGGGGGGGGGGG267020.11831483727965733TruSeq Adapter, Index 8 (97% over 41bp)
TATGTCCGGGCTATAGAGAGAACCTCTTCATAGATCGGAAGAGCACACGT263960.11695897104463467No Hit
GTGTAATGATGTCCATCTTTCGGGTCAGAATTTTCATGTTGACAAATCTC245940.1089744254383901No Hit
TAAGACTGTATCTGAAGCTATGTTGGGCATAGATCGGAAGAGCACACGTC242700.10753880236601317No Hit
TACGTGAAATCGCTCAGGACTTCAAGACTGAGATCGGAAGAGCACACGTC234990.10412255116600509No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)