FastQCFastQC Report
Thu 5 Dec 2024
71_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename71_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21953712
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGT2742651.2492875920026645Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGTG2567211.1693739992580754Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCAGGCCGGGACAAGTGCAATTGATCGTCGGACTGTAGAACTCTGAACGT1304980.5944233940939009Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCTCGATTATTGCAGCAGATCCATTTAGAGATCGTCGGACTGTAGAACT1166830.5314955393420484No Hit
ACGTCTAATAATTCTGATACCAAACTACAGATCGTCGGACTGTAGAACTC962600.4384679911989371Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACAACAAAATCACAAGTCTTCCAGATCGTCGGACTGTAGAACTCTGAACG862710.39296771315939644Illumina Small RNA Adapter 1 (100% over 26bp)
TCACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACG813400.3705068190746057Illumina Small RNA Adapter 1 (100% over 26bp)
GAACTACAATATGAAATGTTCAGATCTACAGATCGTCGGACTGTAGAACT798750.3638336878975182No Hit
AAGATGTTAAAGTTCATCAATAGTTCGATAGATCGTCGGACTGTAGAACT757540.34506237487309666No Hit
GATAACCAGCTAACTCCGGACCACATGATCGTCGGACTGTAGAACTCTGA740130.33713205311247596Illumina DpnII expression Sequencing Primer (100% over 24bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT605710.27590322766373176Illumina RNA PCR Primer (100% over 50bp)
TATGTTCCAAATCCATAATTCAGATCGTCGGACTGTAGAACTCTGAACGT589370.2684602950061475Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCTTCTATAGACTACAATTCGGCAGATCGTCGGACTGTAGAACTCTGAAC540050.24599484588301057Illumina DpnII expression Sequencing Primer (100% over 26bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA537290.24473765529947739Illumina RNA PCR Primer (100% over 45bp)
GGAAGCTCCCAGGTGAGTCGAAGATCGTCGGACTGTAGAACTCTGAACGT536770.24450079330547836Illumina DpnII expression Sequencing Primer (96% over 28bp)
GTGGCGGTCAAGGGACACATACGGCAAGGATCGTCGGACTGTAGAACTCT468010.21318034963745536Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACGTCTAATAATTCTGACACCAAACTACAGATCGTCGGACTGTAGAACTC400250.1823154098040459Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGCAATTCATAGATAACCAGCTAACTCCGGACCACATGATCGTCGGACTG357130.16267408445551257No Hit
GCAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGTG340190.1549578494971602Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCAAGAGATTAGCAGACACAGATTTACTAGATCGTCGGACTGTAGAACTC339610.1546936572730844Illumina DpnII expression Sequencing Primer (100% over 21bp)
TACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGT335480.1528124264361307Illumina DpnII expression Sequencing Primer (96% over 28bp)
ATAACCAGCTAACTCCGGACCACATGATCGTCGGACTGTAGAACTCTGAA334000.15213828076090274Illumina DpnII expression Sequencing Primer (100% over 25bp)
CGTCCCAAATCCCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC332020.15123638316836804Illumina RNA PCR Primer (100% over 30bp)
ACAGGCCGGGACAAGTGCAATTGATCGTCGGACTGTAGAACTCTGAACGT320780.14611652006731254Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG310320.14135194995725553Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACCTCGATTATTGCAGCAGATCCATTTAGAGATCGTCGGACTGTAGAACT304000.13847316572249832No Hit
CATGATTGTGTGTATTTCAGAGTTCGTTCAGATCGTCGGACTGTAGAACT283800.129271988263306No Hit
ATTTTTTTCCTAACCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA281500.128224329443695Illumina DpnII expression Sequencing Primer (96% over 30bp)
CAAGCTCTAAAAACAGCCAAGAGATCAGAGATCGTCGGACTGTAGAACTC270240.12309535626594718Illumina DpnII expression Sequencing Primer (100% over 21bp)
CATAGATAACCAGCTAACTCCGGACCACATGATCGTCGGACTGTAGAACT267650.12191560133429827No Hit
ATCTCACTACCCATGTCTTTCAGATCGTCGGACTGTAGAACTCTGAACGT248430.11316081763302717Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTCTACTGTGATAACCACTACACTAATCCGGCGATCGTCGGACTGTAGA237540.10820038087408636No Hit
CAGTCTTGAAGTCCTGAGCGATTTCACGTAGATCGTCGGACTGTAGAACT237510.10818671575904795No Hit
GCCTCGATTGTTGCAGCAGATCCATTTAGAGATCGTCGGACTGTAGAACT235380.10721649259132122No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)