FastQCFastQC Report
Thu 5 Dec 2024
71_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename71_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21953712
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC66

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC2638121.2016737761705172Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA2479331.1293443222722426Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACGTCTGAACTCC1285290.5854545235903613Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTAAATGGATCTGCTGCAATAATCGAGGCAGATCGGAAGAGCACACGTC1163160.5298238402690169No Hit
TGTAGTTTGGTATCAGAATTATTAGACGTAGATCGGAAGAGCACACGTCT923340.42058491065201187Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGAAGACTTGTGATTTTGTTGTAGATCGGAAGAGCACACGTCTGAACTC821190.3740551939462447Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTAGATCTGAACATTTCATATTGTAGTTCAGATCGGAAGAGCACACGTC787090.358522513185925No Hit
AACCCGTAGATCCGAACTTGTGAAGATCGGAAGAGCACACGTCTGAACTC786680.35833575661373346Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TATCGAACTATTGATGAACTTTAACATCTTAGATCGGAAGAGCACACGTC734560.3345948967536788No Hit
ATGTGGTCCGGAGTTAGCTGGTTATCAGATCGGAAGAGCACACGTCTGAA731670.33327849067164583Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGAATTATGGATTTGGAACATAAGATCGGAAGAGCACACGTCTGAACTCC560470.25529623418581787Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCGACTCACCTGGGAGCTTCCAGATCGGAAGAGCACACGTCTGAACTCC536470.24436414215509433Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGCCGAATTGTAGTCTATAGAAGCAGATCGGAAGAGCACACGTCTGAACT525740.23947658600969166Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTGCCGTATGTGTCCCTTGACCGCCACAGATCGGAAGAGCACACGTCTG466750.21260641480584241Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTAGTTTGGTGTCAGAATTATTAGACGTAGATCGGAAGAGCACACGTCT380910.17350596564262116Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGTGGTCCGGAGTTAGCTGGTTATCTATGAATTGCCAGATCGGAAGAGC360110.16403148588266075No Hit
AACCCGTAGATCCGAACTTGCAGATCGGAAGAGCACACGTCTGAACTCCA342550.15603283854684802Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGTAAATCTGTGTCTGCTAATCTCTTGCAGATCGGAAGAGCACACGTCT333800.15204717999398007Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACCCGTAGATCCGAACTTGTAAGATCGGAAGAGCACACGTCTGAACTCC328190.1494918034817984Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATGTGGTCCGGAGTTAGCTGGTTATAGATCGGAAGAGCACACGTCTGAAC323540.14737371065084573Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGGGATTTGGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC315860.14387544120101423Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC297510.13551694583585683Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA296650.13512521253808923Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTAAATGGATCTGCTGCAATAATCGAGGTAGATCGGAAGAGCACACGTC286630.13056106411526214No Hit
TGAACGAACTCTGAAATACACACAATCATGAGATCGGAAGAGCACACGTC272840.12427966623594223No Hit
TCGGTTAGGAAAAAAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA269170.12260796716291077Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCTGATCTCTTGGCTGTTTTTAGAGCTTGAGATCGGAAGAGCACACGTCT259930.1183991117310822Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGTGGTCCGGAGTTAGCTGGTTATCTATGAGATCGGAAGAGCACACGTC257640.11735600794981732No Hit
TGAAAGACATGGGTAGTGAGATAGATCGGAAGAGCACACGTCTGAACTCC237840.1083370320244704Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTAAATGGATCTGCTGCAACAATCGAGGCAGATCGGAAGAGCACACGTC233780.10648768645593967No Hit
TACGTGAAATCGCTCAGGACTTCAAGACTGAGATCGGAAGAGCACACGTC228880.1042557176663336No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)