FastQCFastQC Report
Thu 5 Dec 2024
501_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename501_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21244405
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAATCTCCTAGTCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA6981413.2862346580193704Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA3968001.8677858946861539Illumina RNA PCR Primer (100% over 45bp)
ACAAATCTCCTAGTCGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAG3412621.606361769134038Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGATCGTC1675020.7884523007351818No Hit
GCTCACTCAAGCCAGCTGTGATAGATCGTCGGACTGTAGAACTCTGAACG1018450.4793968105955427Illumina Small RNA Adapter 1 (100% over 26bp)
CAATATCTGAGACATCTGGAAGAACTACAGATCGTCGGACTGTAGAACTC929350.43745635615589135Illumina DpnII expression Sequencing Primer (100% over 21bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT798120.37568479795033094Illumina RNA PCR Primer (100% over 50bp)
GCAAATCTCCTAGTCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA720470.339133997869086Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACCCTTATCAGTTCTCCGTCCAGATCGTCGGACTGTAGAACTCTGAACGT677470.3188933745143721Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGGATCGT661450.3113525655343136No Hit
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGTGATCG498080.2344523181515321No Hit
ACGTCTGATCCGAGGTCGGAGATCGTCGGACTGTAGAACTCTGAACGTGT477420.22472740469784866Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAGATGTTAAAGTTCATCAATAGTTCGATAGATCGTCGGACTGTAGAACT449910.21177811287254222No Hit
ACTCACTCAAGCCAGCTGTGATAGATCGTCGGACTGTAGAACTCTGAACG346270.1629935034659714Illumina Small RNA Adapter 1 (100% over 26bp)
GCAAATCTCCTAGTCGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAG346150.1629370180054466Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGGTTGCAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACT339530.15982090343316274No Hit
ACGGAATTATCTATTTCCCAGAAGATCACAGATCGTCGGACTGTAGAACT325960.1534333392721519No Hit
CCAGGTGTAAGCACCAAAAGACCGATACAGATCGTCGGACTGTAGAACTC323670.15235540840047063Illumina DpnII expression Sequencing Primer (100% over 21bp)
CCGTCTGATCCGAGGTCGGAGATCGTCGGACTGTAGAACTCTGAACGTGT317260.14933814338410514Illumina DpnII expression Sequencing Primer (96% over 30bp)
CAGACAAAATGGATAATCTAGAGATCTTAGATCGTCGGACTGTAGAACTC312650.1471681602756114Illumina DpnII expression Sequencing Primer (100% over 21bp)
GACTTGTTCATATTGAGTTATTCAGATCTGAGATCGTCGGACTGTAGAAC308430.14518175491382318No Hit
AATATAATTGATTCTACACAACAATATACTTAAGATCGTCGGACTGTAGA295370.13903425396004265No Hit
AAGATGTTAAGATTCATCAATAGTTCGATAGATCGTCGGACTGTAGAACT278940.1313004529898578No Hit
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGTGGATC272580.1283067235820443No Hit
GTTCAGTGAGATTTGTCAACATGAAAATTCTGACCCGAAAGATGGACATC265030.12475284669069338No Hit
AAAAATATAGGTCAAAGTCAATTACGTCAAGATCGTCGGACTGTAGAACT234360.11031610440490096No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)