FastQCFastQC Report
Thu 5 Dec 2024
501_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename501_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21244405
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACTAGGAGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA6334022.981500305609877Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCGACTAGGAGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC3075051.4474634615561133Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGGGGG2470591.162936782649361TruSeq Adapter, Index 20 (97% over 43bp)
ACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATCGG1617990.7616075856207788No Hit
TATCACAGCTGGCTTGAGTGAGCAGATCGGAAGAGCACACGTCTGAACTC1001160.4712581971582636Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTAGGGGG979920.461260270645377TruSeq Adapter, Index 20 (97% over 43bp)
TGTAGTTCTTCCAGATGTCTCAGATATTGAGATCGGAAGAGCACACGTCT836400.3937036598577367Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTAGGAGATTTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA713690.3359425693494357Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATCG644810.3035199150082104No Hit
TGGACGGAGAACTGATAAGGGTAGATCGGAAGAGCACACGTCTGAACTCC615870.28989750477831694Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAACTCTATGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAGCGCAGC493090.23210346441804325No Hit
ACACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATC487130.2292980198786457No Hit
TCCGACCTCGGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG434040.20430791071813967Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATCGAACTATTGATGAACTTTAACATCTTAGATCGGAAGAGCACACGTC417930.19672473764268758No Hit
TTCGACTAGGAGATTTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC342270.16111065478181197Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGATCTGAACTTTTTATCACATTGCAACCCAGATCGGAAGAGCACACGTC338850.15950081915685566No Hit
TGTGATCTTCTGGGAAATAGATAATTCCGTAGATCGGAAGAGCACACGTC303340.14278582996323033No Hit
TCAGATCTGAATAACTCAATATGAACAAGTCAGATCGGAAGAGCACACGT298700.14060172548960537No Hit
GTGTAATGATGTCCATCTTTCGGGTCAGAATTTTCATGTTGACAAATCTC294200.138483520719926No Hit
TGTATCGGTCTTTTGGTGCTTACACCTGGAGATCGGAAGAGCACACGTCT290480.13673247144365774Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATCACAGCTGGCTTGAGTGAGTAGATCGGAAGAGCACACGTCTGAACTC287280.13522619249633022Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAAGATCTCTAGATTATCCATTTTGTCTGAGATCGGAAGAGCACACGTCT282270.1328679245194205Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGGGGGGGGGG280640.13210066368062556TruSeq Adapter, Index 20 (97% over 39bp)
TCCGACCTCGGATCAGACGGAGATCGGAAGAGCACACGTCTGAACTCCAG279690.13165348711813768Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CACACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGAT264670.12458339030911904No Hit
TTAAGTATATTGTTGTGTAGAATCAATTATATTAGATCGGAAGAGCACAC263940.12423977042425995No Hit
TATCGAACTATTGATGAATCTTAACATCTTAGATCGGAAGAGCACACGTC261160.12293119058876913No Hit
TTGACGTAATTGACTTTGACCTATATTTTTAGATCGGAAGAGCACACGTC218310.10276117405971126No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)