FastQCFastQC Report
Thu 5 Dec 2024
485_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename485_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19696678
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAATCTCCTAGTCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA9811734.981413617057658Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA4945552.5108548761369813Illumina RNA PCR Primer (100% over 45bp)
ACAAATCTCCTAGTCGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAG4374312.2208364273406915Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGATCGTC2546031.2926189888467488No Hit
AAGATGTTAAAGTTCATCAATAGTTCGATAGATCGTCGGACTGTAGAACT1645790.8355672971858503No Hit
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGGATCGT1291380.6556334017340386No Hit
GCAAATCTCCTAGTCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA1065570.5409897039490619Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT996530.5059381079388108Illumina RNA PCR Primer (100% over 50bp)
GCTCACTCAAGCCAGCTGTGATAGATCGTCGGACTGTAGAACTCTGAACG973030.4940071620199102Illumina Small RNA Adapter 1 (100% over 26bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGTGATCG755620.3836281427761575No Hit
ACCCTTATCAGTTCTCCGTCCAGATCGTCGGACTGTAGAACTCTGAACGT490690.2491232277849087Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGGTTGCAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACT479270.24332529576814935No Hit
GCAAATCTCCTAGTCGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAG467620.23741059279133261Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCTTTTACAACCGAAAGAAACACAACGTAGATCGTCGGACTGTAGAACTC443930.22538318390542814Illumina DpnII expression Sequencing Primer (100% over 21bp)
CAGTCTTGAAGTCCTGAGCGATTTCACGTAGATCGTCGGACTGTAGAACT333580.16935850806922872No Hit
GCTATAAAGACAACTTTGCAGACCAAATAGATCGTCGGACTGTAGAACTC328040.1665458510313262Illumina DpnII expression Sequencing Primer (100% over 21bp)
CAATATCTGAGACATCTGGAAGAACTACAGATCGTCGGACTGTAGAACTC323270.16412412285970254Illumina DpnII expression Sequencing Primer (100% over 21bp)
AACAAATCTCCTAGTCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAG292670.1485885081738149Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGATCGTC275250.13974437719903834No Hit
AAATTGTTGAAGCTTTAGACCGAGTATTCAGATCGTCGGACTGTAGAACT271460.13782019485722416No Hit
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGTGGATC271080.1376272689232164No Hit
ACTCACTCAAGCCAGCTGTGATAGATCGTCGGACTGTAGAACTCTGAACG268500.136317403371269Illumina Small RNA Adapter 1 (100% over 26bp)
ATATTCACAGATCCTGATGATATAGCCAGATCGTCGGACTGTAGAACTCT251910.1278946632523515Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGGTTGCAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACT219090.11123195495199749No Hit
ATGCCCAACATAGCTTCAGATACAGTCTTAGATCGTCGGACTGTAGAACT215000.10915546266228243No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)