FastQCFastQC Report
Thu 5 Dec 2024
485_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename485_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19696678
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC66

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACTAGGAGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA8860754.4986012362084615Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCGACTAGGAGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC3923511.991965345628334Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTGGGGGG3198521.6238880485328542TruSeq Adapter, Index 19 (97% over 43bp)
ACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATCGG2457821.2478347871656328No Hit
TATCGAACTATTGATGAACTTTAACATCTTAGATCGGAAGAGCACACGTC1535680.7796644693079716No Hit
CACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATCG1258490.638935154445841No Hit
TCGACTAGGAGATTTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1038380.5271853456709806Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TATCACAGCTGGCTTGAGTGAGCAGATCGGAAGAGCACACGTCTGAACTC940170.4773241457265027Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTAGGGGG821390.41701956035428916TruSeq Adapter, Index 19 (97% over 43bp)
ACACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATC738160.37476370380832746No Hit
CAACTCTATGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAGCGCAGC531810.2699998446438531No Hit
TGATCTGAACTTTTTATCACATTGCAACCCAGATCGGAAGAGCACACGTC465770.23647134811261064No Hit
TTCGACTAGGAGATTTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC452690.2298306343841332Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGGACGGAGAACTGATAAGGGTAGATCGGAAGAGCACACGTCTGAACTCC444210.22552533985680223Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACGTTGTGTTTCTTTCGGTTGTAAAAGCAGATCGGAAGAGCACACGTCT416440.21142651567944604Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATTTGGTCTGCAAAGTTGTCTTTATAGCAGATCGGAAGAGCACACGTCT307280.15600600263658673Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACGTGAAATCGCTCAGGACTTCAAGACTGAGATCGGAAGAGCACACGTC303560.15411735928261608No Hit
TGTAGTTCTTCCAGATGTCTCAGATATTGAGATCGGAAGAGCACACGTCT290630.14755280052808906Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGCAGATCGG277730.1410034727683521No Hit
TCGACTAGGAGATTTGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTC264820.1344490680103518Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CACACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGAT263450.1337535192482712No Hit
TGAATACTCGGTCTAAAGCTTCAACAATTTAGATCGGAAGAGCACACGTC251570.1277220453113972No Hit
TATCACAGCTGGCTTGAGTGAGTAGATCGGAAGAGCACACGTCTGAACTC244760.12426460949404768Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGCTATATCATCAGGATCTGTGAATATAGATCGGAAGAGCACACGTCTG230070.11680649904516893Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTGGGGGGGGGGG226150.11481631572593104TruSeq Adapter, Index 19 (97% over 39bp)
TGATCTGAACTTTTTATCACATTGCAACCTAGATCGGAAGAGCACACGTC202780.10295137078445411No Hit
TAAGACTGTATCTGAAGCTATGTTGGGCATAGATCGGAAGAGCACACGTC199650.10136227032802182No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTGGGGTG199340.10120488338185758TruSeq Adapter, Index 19 (97% over 43bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)