FastQCFastQC Report
Thu 5 Dec 2024
460_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename460_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18956830
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAATCTCCTAGTCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA8533694.5016439984955285Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAATCTCCTAGTCGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAG4105692.1658104229451864Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA3846522.0290945268802854Illumina RNA PCR Primer (100% over 45bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGATCGTC2751741.451582358442841No Hit
GCTCACTCAAGCCAGCTGTGATAGATCGTCGGACTGTAGAACTCTGAACG863700.4556141506781461Illumina Small RNA Adapter 1 (100% over 26bp)
GCAAATCTCCTAGTCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA857770.4524859905374474Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGGATCGT706850.3728735236851309No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT674790.35596141337976867Illumina RNA PCR Primer (100% over 50bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGTGATCG644430.3399460774823639No Hit
ACCCTTATCAGTTCTCCGTCCAGATCGTCGGACTGTAGAACTCTGAACGT464850.2452150491406Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCAAATCTCCTAGTCGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAG411650.21715128531510805Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGTGGATC397590.20973443344694237No Hit
GTGCTCTTGTAATGAGGAGCTTCAGACCTTAGATCGTCGGACTGTAGAAC350930.1851206135202985No Hit
GCTTCTATAGACTACAATTCGGCAGATCGTCGGACTGTAGAACTCTGAAC331630.17493958641819335Illumina DpnII expression Sequencing Primer (100% over 26bp)
AATATGATTGATTCTACACAACAATATACTTAAGATCGTCGGACTGTAGA284380.15001453302055248No Hit
GCAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGATCGTC276880.14605817533838727No Hit
ACAAATCTCCTAGTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT263990.13925851526863933Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACTCACTCAAGCCAGCTGTGATAGATCGTCGGACTGTAGAACTCTGAACG259270.13676864750066337Illumina Small RNA Adapter 1 (100% over 26bp)
AGTCTACTGTGATAACCACTACACTAATCCGGCGATCGTCGGACTGTAGA251910.13288614182856523No Hit
CAATCGCATCAAGATCTTCAACGTCTTCAGATCGTCGGACTGTAGAACTC227260.11988291291318222Illumina DpnII expression Sequencing Primer (100% over 21bp)
CAGACAAAATGGATAATCTAGAGATCTTAGATCGTCGGACTGTAGAACTC221100.11663342447023053Illumina DpnII expression Sequencing Primer (100% over 21bp)
AAAAGCTGATAACCAGTTCGACCAATGATCGTCGGACTGTAGAACTCTGA215560.1137109949290045Illumina DpnII expression Sequencing Primer (100% over 24bp)
GCTCATCAAAGCAGGCTGTGATAGATCGTCGGACTGTAGAACTCTGAACG212580.11213900214329083Illumina Small RNA Adapter 1 (100% over 26bp)
CATGCCATCCAGATGGCGAACCGAGACAAAGATCGTCGGACTGTAGAACT208510.10999201870776917No Hit
GTATACAATGTGTACGACAGTTTCTCGGAGATCGTCGGACTGTAGAACTC198190.10454807053710985Illumina DpnII expression Sequencing Primer (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)