FastQCFastQC Report
Thu 5 Dec 2024
460_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename460_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18956830
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC69

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACTAGGAGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA7726434.07580275816157Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCGACTAGGAGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC3696451.9499304472319474Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATCGG2652481.3992212833052784No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTGGGGGG2013411.0621026827797686TruSeq Adapter, Index 18 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTAGGGGG1152840.6081396520409794TruSeq Adapter, Index 18 (97% over 45bp)
TCGACTAGGAGATTTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA847270.4469470897824162Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TATCACAGCTGGCTTGAGTGAGCAGATCGGAAGAGCACACGTCTGAACTC846500.44654090372704713Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATCG685870.36180627246222075No Hit
ACACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATC629160.3318909332414755No Hit
CAACTCTATGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAGCGCAGC550360.2903228018608596No Hit
TGGACGGAGAACTGATAAGGGTAGATCGGAAGAGCACACGTCTGAACTCC422650.22295394324895038Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCGACTAGGAGATTTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC405000.2136433148369216Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CACACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGAT386180.20371549462647498No Hit
TAAGGTCTGAAGCTCCTCATTACAAGAGCACAGATCGGAAGAGCACACGT347110.18310550867418235No Hit
TGCCGAATTGTAGTCTATAGAAGCAGATCGGAAGAGCACACGTCTGAACT310670.16388288548243563Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGCAGATCGG280560.14799942817443634No Hit
TTAAGTATATTGTTGTGTAGAATCAATCATATTAGATCGGAAGAGCACAC254380.13418910229189163No Hit
CGACTAGGAGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC239140.12614978348173192Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTGTAATGATGTCCATCTTTCGGGTCAGAATTTTCATGTTGACAAATCTC226400.11942925056562727No Hit
TATCACAGCTGGCTTGAGTGAGTAGATCGGAAGAGCACACGTCTGAACTC217350.11465524562914792Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTGGGGGGGGGGG211080.11134773060685779TruSeq Adapter, Index 18 (97% over 41bp)
TGAAGACGTTGAAGATCTTGATGCGATTGAGATCGGAAGAGCACACGTCT204470.10786086070297618Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATCACAGCCTGCTTTGATGAGCAGATCGGAAGAGCACACGTCTGAACTC200150.10558199867804902Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAAGATCTCTAGATTATCCATTTTGTCTGAGATCGGAAGAGCACACGTCT199080.10501755831539344Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTGGTCGAACTGGTTATCAGCTTTTAGATCGGAAGAGCACACGTCTGAA197970.10443201737843301Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCGAGAAACTGTCGTACACATTGTATACAGATCGGAAGAGCACACGTCT197340.10409968333313113Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTGTCTCGGTTCGCCATCTGGATGGCATGAGATCGGAAGAGCACACGTC190580.1005336862756062No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)