FastQCFastQC Report
Thu 5 Dec 2024
376_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename376_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36539657
Total Bases5.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAATCTCCTAGTCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA16122274.412266376775239Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAATCTCCTAGTCGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAG7466382.0433634612388394Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA7435702.0349671043710127Illumina RNA PCR Primer (100% over 45bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGATCGTC4871671.3332555365804337No Hit
ACGTCTAATAATTCTGATACCAAACTACAGATCGTCGGACTGTAGAACTC2047760.560421243144127Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGGATCGT1903860.5210393737412478No Hit
GCAAATCTCCTAGTCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA1694110.46363598870126227Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT1657140.45351821447037666Illumina RNA PCR Primer (100% over 50bp)
AATTTTTACAGTGCTTAAATCATGAAGGAGATCGTCGGACTGTAGAACTC1350660.3696422218741681Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCTCACTCAAGCCAGCTGTGATAGATCGTCGGACTGTAGAACTCTGAACG1347760.3688485636304687Illumina Small RNA Adapter 1 (100% over 26bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGTGATCG1232770.33737864589150357No Hit
GTATACAATGTGTACAACAGTTTCTCGGAGATCGTCGGACTGTAGAACTC804580.22019363783299883Illumina DpnII expression Sequencing Primer (100% over 21bp)
CCAGGTGTAAGCACCAAAAGACCGATACAGATCGTCGGACTGTAGAACTC791780.21669059455046336Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCAAATCTCCTAGTCGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAG767690.21009775762262903Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACCCTTATCAGTTCTCCGTCCAGATCGTCGGACTGTAGAACTCTGAACGT740000.2025196897715816Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACGGAATTATCTATTTCCCAGAAGATCACAGATCGTCGGACTGTAGAACT649600.17777944658867487No Hit
AATCTTCATAAAGAACACAAGAGACATAGAGATCGTCGGACTGTAGAACT595130.1628723553699478No Hit
CCATCATGAAAACTGGAAGAGTTTAGTCAGATCGTCGGACTGTAGAACTC534310.14622742627277535Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCCTTCATTGAGGTCAAAACAGAGCACAAAGATCGTCGGACTGTAGAACT532710.14578954586245843No Hit
ACACGTCAGGAAGTCATAAAATTATTGAAGATCGTCGGACTGTAGAACTC520750.14251638979533934Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGATCGTC506740.1386821994525017No Hit
CCTGTAAACAAATACTACTTGAGATCTAAGATCGTCGGACTGTAGAACTC478280.13089340165398927Illumina DpnII expression Sequencing Primer (100% over 21bp)
CAGACAAAATGGATAATCTAGAGATCTTAGATCGTCGGACTGTAGAACTC477080.13056499134625155Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGTGGATC472770.12938545099096033No Hit
AAATTGTTGAAGCTTTAGACCGAGTATTCAGATCGTCGGACTGTAGAACT446250.12212758318995716No Hit
ACTCACTCAAGCCAGCTGTGATAGATCGTCGGACTGTAGAACTCTGAACG400350.10956588891899012Illumina Small RNA Adapter 1 (100% over 26bp)
AAGATGTTAAAGTTCATCAATAGTTCGATAGATCGTCGGACTGTAGAACT388260.10625715506853281No Hit
CTTTGATCGTGAAGAGTTTGCTATTAGTTAGATCGTCGGACTGTAGAACT379930.10397744018232026No Hit
CAATCGCATCAAGATCTTCAACGTCTTCAGATCGTCGGACTGTAGAACTC371590.10169498854354325Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACAAATCTCCTAGTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT371020.10153899364736783Illumina DpnII expression Sequencing Primer (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)