FastQCFastQC Report
Thu 5 Dec 2024
376_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename376_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36539657
Total Bases5.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACTAGGAGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA14584253.991348358852958Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCGACTAGGAGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC6709831.8363144459730425Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGGG6642531.8178961012140866TruSeq Adapter, Index 3 (97% over 37bp)
ACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATCGG4711571.2894401280230956No Hit
TGTAGTTTGGTATCAGAATTATTAGACGTAGATCGGAAGAGCACACGTCT1876590.5135762494979085Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATCG1859930.5090168197254835No Hit
TCGACTAGGAGATTTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1662220.45490848477313295Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TATCACAGCTGGCTTGAGTGAGCAGATCGGAAGAGCACACGTCTGAACTC1304060.35688895492368744Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTTCATGATTTAAGCACTGTAAAAATTAGATCGGAAGAGCACACGTCT1251090.3423923765896325Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGATC1207660.3305066602020922No Hit
TCCGAGAAACTGTTGTACACATTGTATACAGATCGGAAGAGCACACGTCT792830.21697795356973382Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCGACTAGGAGATTTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC749000.20498276707961435Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGGGGGGGG735510.2012908878701297TruSeq Adapter, Index 3 (96% over 33bp)
TGTATCGGTCTTTTGGTGCTTACACCTGGAGATCGGAAGAGCACACGTCT709580.19419448847043091Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGACGGAGAACTGATAAGGGTAGATCGGAAGAGCACACGTCTGAACTCC671310.18372093640616277Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAACTCTATGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAGCGCAGC662710.18136732920070925No Hit
TGTGATCTTCTGGGAAATAGATAATTCCGTAGATCGGAAGAGCACACGTC602520.1648948155150991No Hit
TCTATGTCTCTTGTGTTCTTTATGAAGATTAGATCGGAAGAGCACACGTC544830.1491064899706092No Hit
ACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGCAGATCGG514320.14075665789637817No Hit
TTTGTGCTCTGTTTTGACCTCAATGAAGGCAGATCGGAAGAGCACACGTC504770.138143059197299No Hit
TGACTAAACTCTTCCAGTTTTCATGATGGAGATCGGAAGAGCACACGTCT482490.13204557448363569Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCAATAATTTTATGACTTCCTGACGTGTAGATCGGAAGAGCACACGTCT479320.13117802392069527Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACACTGCGATCTATTGAGACTAAGCCTTTCGACTAGGAGATTTGTAGAT459860.12585230343021556No Hit
TAAGATCTCTAGATTATCCATTTTGTCTGAGATCGGAAGAGCACACGTCT429950.11766667650985338Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTAGATCTCAAGTAGTATTTGTTTACAGGAGATCGGAAGAGCACACGTCT428330.1172233225944075Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAATACTCGGTCTAAAGCTTCAACAATTTAGATCGGAAGAGCACACGTC414090.11332618694258678No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)