FastQCFastQC Report
Thu 5 Dec 2024
367_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename367_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19352690
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAATCTCCTAGTCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA8459164.371051259540663Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA5478212.8307227574047844Illumina RNA PCR Primer (100% over 45bp)
ACAAATCTCCTAGTCGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAG3579211.8494638213085624Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGATCGTC2435071.2582591877408258No Hit
GGGTTGCAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACT1330240.687366975857103No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT1140260.5891997443249491Illumina RNA PCR Primer (100% over 50bp)
GCAAATCTCCTAGTCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA936170.4837415367062667Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCTCACTCAAGCCAGCTGTGATAGATCGTCGGACTGTAGAACTCTGAACG767470.3965701925675449Illumina Small RNA Adapter 1 (100% over 26bp)
AGGTTGCAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACT590970.30536840098198237No Hit
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGGATCGT574750.29698713718868025No Hit
GGCTTAAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACTC514170.2656839953515506Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGTGATCG501220.2589924191417317No Hit
CCAGGTGTAAGCACCAAAAGACCGATACAGATCGTCGGACTGTAGAACTC446010.23046408535454246Illumina DpnII expression Sequencing Primer (100% over 21bp)
AAGATGTTAAAGTTCATCAATAGTTCGATAGATCGTCGGACTGTAGAACT421180.21763382764876613No Hit
ACCCTTATCAGTTCTCCGTCCAGATCGTCGGACTGTAGAACTCTGAACGT401280.20735101941900583Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAATATCTGAGACATCTGGAAGAACTACAGATCGTCGGACTGTAGAACTC394260.20372361671684916Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCAAATCTCCTAGTCGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAG390310.20168255679184652Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGTGGATC368300.1903094608553126No Hit
GCTATAAAGACAACTTTGCAGACCAAATAGATCGTCGGACTGTAGAACTC338140.17472506406086183Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCTTTGGACATCATTGTCTTAACGATCGGAGATCGTCGGACTGTAGAACT332360.1717383991579465No Hit
AAATTGTTGAAGCTTTAGACCGAGTATTCAGATCGTCGGACTGTAGAACT281810.14561799935822875No Hit
CAGACAAAATGGATAATCTAGAGATCTTAGATCGTCGGACTGTAGAACTC279570.14446053752734117Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGATCGTC263860.13634280299017862No Hit
GGGTTGCAATGTAATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACT244640.12641136710193776No Hit
CACGAGCCGAGTGATCCACCGCATAGAGTTGGATCGTCGGACTGTAGAAC226190.11687780871806452No Hit
CAGTCTTGAAGTCCTGAGCGATTTCACGTAGATCGTCGGACTGTAGAACT222230.11483158155274538No Hit
ACTCACTCAAGCCAGCTGTGATAGATCGTCGGACTGTAGAACTCTGAACG207250.1070910555586846Illumina Small RNA Adapter 1 (100% over 26bp)
AGCTTAAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACTC198330.10248187719640008Illumina DpnII expression Sequencing Primer (100% over 21bp)
GTATACAATGTGTACAACAGTTTCTCGGAGATCGTCGGACTGTAGAACTC193840.10016178629430844Illumina DpnII expression Sequencing Primer (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)